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1 ml Control DNA Template 0.1 µg/µl in 10 mM Tris-HCl, 1 mM EDTA, pH 8 10 µl Control PCR Primers 0.1 µg/µl each in 10 mM Tris-HCl, 1 mM EDTA, pH 8 10 µl One Shot®Reagents The table below describes the items incl...
With a Ct below 40, PET-PCR was able to detect less than 0.2 parasite/µL of blood, which meets the WHO’s Evidence Review Group recommendation of at least 2 parasites/µL for molecular assays [40]. However, 15 cases showed a Ct above 40 in both replicates, which could be real ...
In validation tests, average yields of approxi- mately 2.9 µg DNA with a mean A260/A280 ratio of 1.7 per sample were obtained (exceeding specifications and equivalent to an average DNA concentration of 72 ng/µl). For qualitative analysis of the plasmid DNA, 3 µl of each purified...
The densities of female and male gametocytes in the blood and the sex ratio (proportion of gametocytes that are male) influence infectivity to mosquitoes, and so, onwards transmission to new hosts [1], [2], [3]. Techniques to determine the sex of P. falciparum gametocytes are laborious. ...
Therefore, we expected to have "susceptible" marker scores for all European, African, and Middle East lines except the Ethiopian parent P27174 (distribution ratio like 106 vs 1). Simple matching coefficients were calculated, to compare the marker and expected pheno- type. These values ranged ...
The filtering capacity of a certain filter is defined as the ratio of filter length and pore size. The longer a filter is, the better it filters. This relation is almost linear. BIOplastics' filters have the longest length available in the market when compared to other brands. On the other...
Total rRNA [3] [4], or total RNA. Drawback: rapidly dividing cells will have more rRNA and different rRNA/mRNA ratio which will complicate comparison; difference incDNAsynthesis not taken into account. Genomic DNA Genomic DNA or cell number. Drawbacks: RNA degrades faster than RNA which can...
The steps are described in more detail below. Step 0: Prepare PCR-Ready Samples prior to Starting ddPCR Step 1: Droplet Generation Prior to droplet generation, nucleic acid samples (DNA or RNA) are prepared as they are for any real-time assay: using primers, fluorescent probes (TaqMan ...
The data for statistically discriminating DNA in the well may include the following items: a fluorescence intensity at a temperature lower than the melting temperature; a fluorescence intensity at a temperature higher than the melting temperature; a ratio of a fluorescence intensity at a temperature ...