PCR is performed in two stages using nested primers; performing the secondary (nested) amplification is absolutely essential for diluting out low-molecular-weight junk and also generating an adequate mass of larger, useful cDNAs for identification purposes. When performed correctly, the primary and se...
from Collinson and Randox will make the PCR testing process far more seamless for passengers, enabling them to book to take their test as part of the journey to the airport. In the next few weeks, the new technology will also be launched at Collinson’s drive-through facility near Gatwick....
Molecular beacons Dual hybridization probes Eclipse probes Amplifluor assays Scorpions PCR primers LUX PCR primers QZyme PCR primers PCR primer- and probe-based detection chemistries share some common features. In general, these chemistries use some form of fluorescence quenching to ensure that ...
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A molecular diagnosis of SARS-CoV-2 is mainly performed using RT-qPCR, which is time-consuming and expensive. Further, patients may experience anxiety, irritability and fear as a result of false positives produced by RT-qPCR. A CRISPR-based approach, on the other hand, eliminates expensive ...
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Molecular identification tools can offer the advantage of a faster turnaround time as the life stage of the specimen is not a limiting factor. Broad detection assays for tephritid fruit flies have been developed, although they do not reliably allow for identification to species level18,19. Microsa...
(-0.983; 95% bootstrap confidence interval, -0.999 to -0.899) between activity of the enzyme drug L-asparaginase and DNA copy number of genes near ... Bussey,K. J. - 《Molecular Cancer Therapeutics》 被引量: 344发表: 2006年 The Role of STAT-3 in the Induction of Apoptosis in Pancreat...
Indirect immunostaining was then completed with anti-mouse IgG Alexa Fluor 488-conjugated secondary antibody (1:1000) (Molecular Probes) for 1 h. Normal mouse IgG antibody was used as a negative control (Millipore). Stained cells were analyzed using a BD FACScalibur II (BD Biosciences). Data ...
et al. Digital PCR for the molecular detection of fetal chromosomal aneuploidy. Proc. Natl. Acad. Sci. USA 104, 13116–13121 (2007). 7. White, R., Blainey, P., Fan, H. & Quake, S. Digital PCR provides sensitive and absolute calibration for high throughput sequencing. BMC Genomics 10...