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Errors observed included not placing the swab or test strip in the provided solution for the full time indicated (n = 11), contamination of the nasal swab before use (n = 9), and improper swabbing technique (n = 8). Concordance between the antigen test and RT-PCR results ...
e, Left, structural alignment of full-length Cas9 proteins, indicating HNH domains exhibit highly flexible orientation. Right, structural alignment of extracted stand-alone HNH domains, indicating a conserved structure of HNH domains in Cas9 proteins. Extended Data Fig. 2 Structural evolutionary ...
Full size image PIK3CA assay results in patients The median cfDNA concentration for the 213 plasma samples was 16 ng/ml of plasma (range 6–1120 ng/ml; mean: 50 ng/ml) (Supplementary Dataset). Sixty-eight patients (32%) harboured at least one mutation. A total of 74 mutations were fou...
(KASP) and genotyping by sequencing (GBS) for quality control analysis in maize Berhanu Tadesse Ertiro1*, Veronica Ogugo2, Mosisa Worku2, Biswanath Das2, Michael Olsen2, Maryke Labuschagne3 and Kassa Semagn2 Abstract Background: Quality control (QC) analysis is an important component in ...
Full size image Effectiveness of the qPCR genotyping system The reference sequence was CH03g12, which presented stable qPCR results in loquat genomes of different ploidies (Fig. 2). The coefficients of variation (CVs) of the Ct values in the three reference sequences for the loquats of each...
Full size table The ability of DNA primers to distinguish between miRNAs with a single base difference was tested for three cases where the one base difference was in the part of the miRNA sequence used for forward primer design and two cases where the difference was in the sequence used for...
In this study, the usefulness of each locus in paternity analysis was assessed by studying false family trios constructed by using an unrelated individual as the putative father. Twenty five meioses in the form of the mother: child pairs in the above trios were analysed for mutations and mode...
The resulting isoforms are likely to be expressed in soluble form, since they lack the transmembrane and intracellular domains. One of these transcripts encodes the full-length sHLA-G1 soluble form and yields a 37-kDa soluble protein that lead to a purified form of sHLA-G and monoclonal ...
Surprisingly, a full, interpretable STR profile was obtained using the Fast PCR protocol and Platinum Taq enzyme (FIGS. 1B to 1C). Studies to compare the sensitivities of AmpliTaq Gold®, Platinum® Taq and SpeedSTAR™ enzymes with the Fast PCR protocol verse the Identifiler Plus kit ...