pcr产物纯化和胶回收试剂盒并不一定是独立分开的,有些品牌的试剂盒可以做到两者共用一套。比如德国MACHEREY-NAGEL品牌的NucleoSpin® Gel and PCR Clean-up试剂盒,就是结合了胶回收和PCR产物纯化的功能。这种试剂盒通过特定的buffer既可以处理凝胶,也可以用于PCR产物纯化,非常方便。
目前有很多品牌的试剂盒都可以做到胶回收和PCR产物纯化共用一套试剂盒。比如来自德国MACHEREY-NAGEL 品牌的NucleoSpin® Gel and PCR Clean-up 试剂盒。 胶和PCR 产物纯化共用一个试剂盒,并不是把两个独立分开的试剂盒组份叠加到一个试剂盒中。这款试剂盒结合 buffer 既可以处理凝胶,也可以用于 PCR 产物纯化。...
Automated PCR clean-up in 96-well plate and 8-well strip format using the MACHEREY-NAGEL NucleoSpin® 8/96 Extract II kits on the epMotion® 5075 from EppendorfIn this application note we demonstrate the integration of the MACHEREY-NAGEL NucleoSpin 8/96 Extract II PCR clean-up kits ...
Buffer NTC 100 mL for clean-up of single stranded DNA 740654.100 德国mn,macherey-nagel,740609.250,NucleoSpin Extract II (250)PCR产物纯化和胶回收上一件macherey-nagel产品:德国mn,macherey-nagel,740923,Buffer BQ1(125 mL) 裂解缓冲液 BQ1下
The CyBi(R)-DiluSpro has been used for semi-automated, ultrafiltration-based PCR product purification with the MACHEREY-NAGEL NucleoFast 96 PCR clean-up kit. The excellent yield (87 %) and the high DNApurity (A260/280 1.7 - 1.8) indicate the suitability of the CyBi(R)-DiluSpro for ...
** Composition of Elution Buffer NE: 5 mM Tris/HCl, pH 8.5 4 MACHEREY-NAGEL – 02/2017, Rev. 04 PCR clean-up, gel extraction 1.2 Reagents, consumables, and equipment to be supplied by user Reagents • 96–100 % ethanol Consumables • 1.5 mL microcentrifuge tubes • Disposable...
For assembly, all the fragments were digested with BsaI-HF v2 (New England Biolabs) and purified directly using NucleoSpin Gel and PCR clean-up (Macherey–Nagel). Then, two adjacent fragments of equimolar amount were mixed and ligated with 400 units of T4 DNA ligase (New England Biolabs) at...
德国mn,macherey-nagel,740658.2,NucleoSpin 96 Extract II (2x96) 纯化PCR产物 NucleoSpin® 8 / 96 Extract II · NucleoSpin® 8 / 96 Extract II Core Kit 特点: 在8 孔条形式或高通量的96 孔板形式纯化回收PCR 产物 节省时间的平行PCR 纯化方法;完全的去除引物与引物二聚体 优化的回收参数;适合在...
another clone was sent for sequencing. If there were no more clones, PCR with modified conditions and/or Gibson assembly was repeated. ETR1 plasmids were prepared using NucleoSpin Plasmid Kit (Macherey-Nagel) and sequenced by the Biological-Medical Research Centre (BMFZ) at the Heinrich Heine ...
DNA was extracted using NucleoSpin Tissue DNA, RNA and protein purification kits (Macherey–Nagel, NZ740952250) following the manufacturer's protocol with the following modifications: the samples were incubated in lysis buffer with Proteinase K at 56 °C overnight, after which the samples were ...