4. Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions, reverse transcribed and subjected to real-time PCR as previously described. [XX] Expression data were normalized to the geometric mean of the housekeeping ...
Each qRT-PCR reaction was performed in triplicate as follows: step 1: denaturation at 95°C for...
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而其她使用者只能对这些文件执行读或者复制得操作.当S a V e as S ay得图标被选中后,当前得检测项目被执行保存操作。当要把一个检测项目保存在某目标文件夹下时,可以选中file菜单下得save as功能键,打 开相关对话框来实现。除了文本洛、建立时间与更新时间会在a ssa第 realplex Mastercycler eD New Assay3 ...
PCR-AS-200 铝膜 100张/5盒/箱 PCR-AS-600 铝膜 100张/10盒/箱 UC-500 超透膜 100张/5盒/箱 n爱思进 PCR-0208-C 0.2ml 八连排透明PCR薄壁管 公司简介 北京杰辉博高生物技术有限公司依托*重点实验室建立,是由具有资深生命科学、生物技术背景及留学归国人员共同创办的高新技术企业。 公司专业经营...
CD133 is recognized as a stem cell marker for normal and cancerous tissues.注意事项:1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。 2. 回收后重复...
[10]Masanori Terashima, et al.ATTRACTION-5: A phase 3 study of nivolumab plus chemotherapy as postoperative adjuvant treatment for pathological stage III (pStage III) gastric or gastroesophageal junction (G/GEJ) cancer.ASCO 2023.Abstract 4000 ...
ABI9700、ABI2720,PTC-200PCR 仪。 【样本要求】 1.适用标本类型:宫颈脱落细胞样本和生殖泌尿道分泌物。 1 2.受检需知 月经正常妇女,在月经来潮后 10~18 天为最佳检查时间; 检查前不要做阴道冲洗,不要在阴道内用药物; 检查前 24 小时内最好避免性生活。
Methods The differential sequence between the X1 and Y1 box of α1 gene was selected as the amplicon of the target gene with β-actin as the reference gene. The specific primers and TaqMan probes were designed, and then a quantitative method for detecting the copy number was established ...
5、hage T4 DNA ligase and 10x bufferReverse transcriptase, required only if RNA is used as a templateThermostable DNA polymerase and 10x amplification buffer as supplied by the manufacturer or homemade 500 mM KCl 100 mM Tris-Cl (pH 8.3, room temperature) 15 mM MgCl2Nucleic Acids/Oligonucleotide...