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About 200 ILP markers were assessed for their potential as PCR-based markers in 24 sorghum genotypes representing different races. Of these, 172 gave clear and robust amplification without multiple amplicons. Forty-eight of these markers were polymorphic yielding 122 alleles with an average of 2.5 ...
The CRISPR system is an adaptive immune system found in prokaryotes that defends host cells against the invasion of foreign DNA1. As part of the ongoing struggle between phages and the bacterial immune system, the CRISPR system has evolved into various t
The reduced form of ZEN, α‐ZEA ralenol, has been used as an anabolic agent for animals and also proposed as hormonal replacement therapy in postmenopausal women. Furthermore, both zearelanol ZEN and derivatives have been patented as oral contraceptives. ZEN has been widely used in the ...
The exponentially amplified products can be treated with an enzyme having exonuclease activity such as T4 DNA polymerase16,17 for efficient cloning of large fragments. Our method can be further used for multiple insertion, deletion and replacement of large DNA fragments not only at a single site ...
对于一台PCR仪器一般很少有用分析灵敏度的说法，因为每一种不同的序列都可以判断为不同的分析物，同一种方法针对不同分析物的LLD、BLD、AS、FS都是不同的，需要精确到不同的应用方向和应用试剂。A：Bio-Rad不同的ddPCR supermix产生的微滴体积都有差别，这就是为什么要在setup实验时，要在软件中...
All except three gave the expected PCR product of about 200 bp. Computer searches of the databases identified the sequence of each of the PCR products analyzed as a laccase gene sequence, suggesting the specificity of the primers. PCR products of the white rot fungi Ganoderma lucidum, Phlebia ...
(5-transmembrane, 5-TM), which specifically localizes to cellular protrusions. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastomas, neuronal and glial stem cells and some other cell types. CD133 is recognized as a stem cell marker for normal and cancerous...
All of the these polymers are readily dissolved in various solvents such as N-methyl-2-pyrrolidinone, N,N-dimethylacetamide and N,N-dimethylformamide and showed glass-transition temperature above 200 degrees C. Thermogravimetric analysis demonstrated that the 10% weight-loss temperatures in nitrogen ...
DNA was extracted and purified from 200 mg starting material for all of the samples, using a cetyltrimethyl-ammonium bromide (CTAB) protocol, with RNase-A and proteinase-K for removal of RNA and protein from the samples, respectively (as described in Annex A.3 of ISO21570:200532), with...