Table 4. Rates of specificity, sensitivity, and false positives calculated for a range of thresholds for both assays. ThresholdUnitsHF183/BacR287hCYTB484 SpecificitySensitivityFalse-positive RateSpecificitySensitivityFalse-positive Rate No amplification (= 0) positive partitions per ddPCR well 0.64 0.59 ...
We also classified all PSD sequences as intact and defective by sequencing, using criteria described by the Pro-Seq IT tool associated with the PSD database, which include intactness thresholds for sequence length, mutations, and deletions.13,36,37 We then compared the two classifications (ddPCR...