This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase Lucia), the human EF-1α promoter and a multiple cloning site in place of the enhancer. It is specifically designed to analyze the effect of methylation on CpG residues present in enhancer elements...
pCpGfree-basic plasmids contain a multiple cloning site (MCS) upstream of the mSEAP or Lucia, a secreted luciferase, reporter gene. Expression of reporter in cells transfected with this plasmid depends on the insertion of a functional promoter or enhancer/promoter cassette upstream from the reporte...
Thus, pCpGfree-basic plasmids allow to study the effect of CpG methylation on a promoter, alone or combined with enhancer elements. • CpG-free plasmid backbone • Reporter plasmid without an enhancer/promoter region: - murine secreted alkaline phosphatase (SEAP) - secreted luciferase (Lucia)...
Mammalian promoter: The CpG-free promoter combines the mouse CMV enhancer, the human elongation factor 1 alpha core promoter and 5’UTR containing a synthetic intron. Polyadenylation signal: The polyadenylation signal is a CpG-free form of the late SV40 polyadenylation signal. ...