作者采用了与原文相同的强力霉素诱导双向系统,驱动bioPROTAC vhhGFP4-SPOP和mCherry报告基因转染/表达。对HEK293 Tet- on 3G细胞进行瞬时转染和强力霉素诱导后,H2B-GFP稳定整合,流式细胞术检测GFP和mCherry荧光。与之前的报告一致,GFP强度在表达vhhGFP4-SPOP的细胞中减少,如mcherry阳性信号。有趣的是,随着vhhGFP4...
为了改变SPOP的底物特异性,使其能够靶向GFP标记的蛋白,其MATH结构域被vhhGFP4取代。作者采用了与原文相同的强力霉素诱导双向系统,驱动bioPROTAC vhhGFP4-SPOP和mCherry报告基因转染/表达。对HEK293 Tet- on 3G细胞进行瞬时转染和强力霉素诱导后,H2B-GFP稳定整合,流式细胞术检测GFP和mCherry荧光。与之前的报告一致,G...
mCherry鼠单抗价格:价格电议产品详情:产品名称: mCherry鼠单抗货号: TAG0080储存环境及条件: PBS, pH 7.4, 50% 甘油,防腐剂 -20°C保存. 避免反复冻融.建议稀释度: W... 包装规格:100ul/500ul/1ml 用途:WB,IP,IF,ELISA 用途:仅供科学研究使用 ...
(D), but with the maximum intensity normalized to 1, calculated as per the formula for nEt(3). (F) Fraction of the RPA1-EGFP/mPCNA-mCherry bound at replication foci when the total cellular pool of RPA1/PCNA is normalized to 1, as per formula (4). (G) Estimated number of RPA ...
Western blots revealed that RPA1-EGFP (hereafter referred to as RPA1) levels were 1:3 those of endogenous RPA1, while mouse PCNA-mCherry (hereafter referred to as PCNA) levels were approximately 1:4.2 those of endogenous PCNA (Figure 1A). Figure 1. Nuclear distribution of PCNA and RPA1 ...