作者采用了与原文相同的强力霉素诱导双向系统,驱动bioPROTAC vhhGFP4-SPOP和mCherry报告基因转染/表达。对HEK293 Tet- on 3G细胞进行瞬时转染和强力霉素诱导后,H2B-GFP稳定整合,流式细胞术检测GFP和mCherry荧光。与之前的报告一致,GFP强度在表达vhhGFP4-SPOP的细胞中减少,如mcherry阳性信号。有趣的是,随着vhhGFP4...
为了改变SPOP的底物特异性,使其能够靶向GFP标记的蛋白,其MATH结构域被vhhGFP4取代。作者采用了与原文相同的强力霉素诱导双向系统,驱动bioPROTAC vhhGFP4-SPOP和mCherry报告基因转染/表达。对HEK293 Tet- on 3G细胞进行瞬时转染和强力霉素诱导后,H2B-GFP稳定整合,流式细胞术检测GFP和mCherry荧光。与之前的报告一致,G...
mCherry鼠单抗价格:价格电议产品详情:产品名称: mCherry鼠单抗货号: TAG0080储存环境及条件: PBS, pH 7.4, 50% 甘油,防腐剂 -20°C保存. 避免反复冻融.建议稀释度: W... 包装规格:100ul/500ul/1ml 用途:WB,IP,IF,ELISA 用途:仅供科学研究使用 ...
Here, in very early S-phase, we show that the fluorescence intensity of mCherry-tagged PCNA after local micro-irradiation was less than the fluorescence intensity of non-irradiated mCherry-PCNA-positive replication foci. However, PCNA protein accumulated at locally irradiated chromatin in very late...
(D), but with the maximum intensity normalized to 1, calculated as per the formula for nEt(3). (F) Fraction of the RPA1-EGFP/mPCNA-mCherry bound at replication foci when the total cellular pool of RPA1/PCNA is normalized to 1, as per formula (4). (G) Estimated number of RPA ...