7. 用00μl 抗体孵育液(0.5% Tween-20/%BSA/PBS)重悬细胞,加入ul PCNA 抗体(μg/06 cells),室温孵育 小时。 8. 离心收集细胞,用50μl% BSA-PBS 洗2 次。 9. 离心收集细胞,用50μl 抗体孵育液重悬,加μl (.5μg/06 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,50μl PBS/ % BSA...
用100μL抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1μL PCNA抗体(1μg/106 cells),室温孵育1小时。 离心收集细胞,用150μL 1% BSA-PBS洗2次。 离心收集细胞,用50μL抗体孵育液重悬,加1μL (1.5μg/106 cells)羊抗鼠IgG-FITC,室温孵育30分钟。离心收集细胞,150μL PBS/1% BSA洗2次。 用...
7. 用100μl 抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1ul PCNA 抗体(1μg/106 cells),室温孵育1 小时。 8. 离心收集细胞,用150μl1% BSA-PBS 洗2 次。 9. 离心收集细胞,用50μl 抗体孵育液重悬,加1μl (1.5μg/106 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,150μl ...
7. 用100μl 抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1ul PCNA 抗体(1μg/106 cells),室温孵育1 小时。 8. 离心收集细胞,用150μl1% BSA-PBS 洗2 次。 9. 离心收集细胞,用50μl 抗体孵育液重悬,加1μl (1.5μg/106 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,150μl ...
英文名PCNA Cell Proliferation Kit(IF) 相关类别毒性与增殖检测储存冷冻(-20℃) 避光 编 号包装库存目录价(￥)您的价格(￥)数量 E607253-0100100 TESTS预计3日内发货25212521 产品描述 概述 The Proliferation Cell Nuclear Antigen (PCNA) is a nuclear antigen in proliferating cells and is a cofactor of DNA...
The Proliferation Cell Nuclear Antigen(PCNA)is a nuclear antigen in proliferating cells and is a cofactor of DNA polymerase delta.PCNA protein levels peak during the S-phase of the cell cycle,at which time it forms a complex with the p21 inhibitor.PCNA is almost undetectable in other phases ...
7. 用100μl 抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1ul PCNA 抗体(1μg/106 cells),室温孵育1 小时。8. 离心收集细胞,用150μl1% BSA-PBS 洗2 次。9. 离心收集细胞,用50μl 抗体孵育液重悬,加1μl (1.5μg/106 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,150μl PBS...
7. 用100μl 抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1ul PCNA 抗体(1μg/106 cells),室温孵育1 小时。 8. 离心收集细胞,用150μl1% BSA-PBS 洗2 次。 9. 离心收集细胞,用50μl 抗体孵育液重悬,加1μl (1.5μg/106 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,150μl ...
PCNA-positive cellsAdult TeleostsAmong heterothermic Vertebrates, only Teleosts have a cerebellum with elements that conserve embryonic characters into adulthood; such cells are present in other encephalic regions but are more abundant in the cerebellum. This is due to the presence in Teleosts of ...
PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458). 实验方案 Product Specific Protocols WB ...