用100μL抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1μL PCNA抗体(1μg/106 cells),室温孵育1小时。 离心收集细胞,用150μL 1% BSA-PBS洗2次。 离心收集细胞,用50μL抗体孵育液重悬,加1μL (1.5μg/106 cells)羊抗鼠IgG-FITC,室温孵育30分钟。离心收集细胞,150μL PBS/1% BSA洗2次。 用...
英文名PCNA Cell Proliferation Kit(IF) 相关类别毒性与增殖检测储存冷冻(-20℃) 避光 编 号包装库存目录价(￥)您的价格(￥)数量 E607253-0100100 TESTS预计3日内发货25212521 产品描述 概述 The Proliferation Cell Nuclear Antigen (PCNA) is a nuclear antigen in proliferating cells and is a cofactor of DNA...
The Proliferation Cell Nuclear Antigen(PCNA)is a nuclear antigen in proliferating cells and is a cofactor of DNA polymerase delta.PCNA protein levels peak during the S-phase of the cell cycle,at which time it forms a complex with the p21 inhibitor.PCNA is almost undetectable in other phases ...
7. 用100μl 抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1ul PCNA 抗体(1μg/106 cells),室温孵育1 小时。 8. 离心收集细胞,用150μl1% BSA-PBS 洗2 次。 9. 离心收集细胞,用50μl 抗体孵育液重悬,加1μl (1.5μg/106 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,150μl ...
7. 用100μl 抗体孵育液(0.5% Tween-20/1%BSA/PBS)重悬细胞,加入1ul PCNA 抗体(1μg/106 cells),室温孵育1 小时。8. 离心收集细胞,用150μl1% BSA-PBS 洗2 次。9. 离心收集细胞,用50μl 抗体孵育液重悬,加1μl (1.5μg/106 cells) 羊抗鼠IgG-FITC,室温孵育30 分钟。离心收集细胞,150μl PBS...
PCNA-positive cellsAdult TeleostsAmong heterothermic Vertebrates, only Teleosts have a cerebellum with elements that conserve embryonic characters into adulthood; such cells are present in other encephalic regions but are more abundant in the cerebellum. This is due to the presence in Teleosts of ...
PCNA Knockout HEK293T Cells (PCNA基因敲除HEK293T细胞)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因,并实现了目的基因CRISPR敲除的HEK293T细胞。本细胞中目的基因的敲除已经通过T7EI法的验证。本细胞株是多克隆细胞,可用于该目的基因的生物学功能研究,也可以用于该基因相应抗体的验证。
12、ytometry and the expression of proliferative markers proliferating cells nuclear antigen and Ki67 in non-Hodgkin's lymphomas.Modern Pathol 1995;8(1):512,Vigliani MC,Chio A,Pezzulo T,et al.Proliferating cell nucler antige (PCNA) in low gradeastrocytomas:its prognostic significance.Tumori 199...
(MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is...
PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458). 实验方案 Product Specific Protocols WB ...