pCMV3-N-HA 载体基本信息 载体名称pCMV3-N-HA 载体大小6041bp 载体类型哺乳动物表达载体 表达方式组成型, 稳定 / 瞬时 启动子CMV 抗生素抗性卡那霉素 在哺乳动物细胞中的筛选潮霉素 标签HA(TATCCTTACGACGTGCCTGACTACGCC) 测序引物Forward: T7(TAATACGACTCACTATAGGG); Reverse: BGH(TAGAAGGCACAGTCGAGG) ...
Vector sequence is the same as pCMV3-N-HA, but multiple cloning sites are removed. Designed for mammalian expression, stable or transient. Hygromycin resistance gene for selection of stable cell lines. pCMV3-N-HA-NCV (Negative Control Vector) Physical Map ...
pCMV3-HA-ULK1质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册 pCMV3-HA-ULK1质粒结构TTTCCTGCGTTATCCCCTGATT...
(His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-...
View All N/C-HA Tag cDNA ORF Clones Expression Vector pCMV3-C-HA Information Description Vector NamepCMV3-C-HA Vector Size6101bp Vector TypeMammalian Expression Vector Expression MethodConstitutive, Stable / Transient PromoterCMV Antibiotic ResistanceKanamycin ...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a ...
View All N/C-HA Tag cDNA ORF Clones Expression Vector pCMV3-C-HA Information Description Vector NamepCMV3-C-HA Vector Size6101bp Vector TypeMammalian Expression Vector Expression MethodConstitutive, Stable / Transient PromoterCMV Antibiotic ResistanceKanamycin ...
pCMV3-ORFSpark- HA-1,HMHA1,HLA-HA1,KIAA0223 pCMV3-ORFSpark- HMP,P87,P89,HCST,IMMT,PIG4,DAP10,KAP10,P87/89,PIK3AP,MGC111146,DKFZP779P1653 pCMV3-ORFSpark- RP3-473B4.1 pCMV3-ORFSpark- STAT5B pCMV3-ORFSpark- DERL3,N27C7-4,C22ORF16,MGC70831 pCMV3-ORFSpark- SPIN1 pCMV3-OR...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a ...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a ...