pCMV3-C-His 载体基本信息 载体名称pCMV3-C-His 载体大小6104bp 载体类型哺乳动物表达载体 表达方式组成型, 稳定 / 瞬时 启动子CMV 抗生素抗性卡那霉素 在哺乳动物细胞中的筛选潮霉素 标签His(CACCATCACCACCATCATCACCACCATCAC) 测序引物Forward: T7(TAATACGACTCACTATAGGG); Reverse: BGH(TAGAAGGCACAGTCGAGG) ...
Myc 标签是来源于 c-myc 基因产物的多肽蛋白质标签,可以使用重组 DNA 技术将其添加到蛋白质中。它可用于亲和层析,从宿主表达的野生型蛋白质中分离重组过表达的蛋白质。它也可以用于分离具有多个亚基的蛋白质复合物。 Myc 标签可用于许多需要抗体识别的不同实验。如果没有针对目的蛋白的抗体,则可以向该蛋白添加 Myc...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a ...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a ...
pCMV3-HA-ULK1质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。
pCMV3-ORFSpark- FGR,SRC2,C-FGR,FLJ43153,P55C-FGR pCMV3-ORFSpark- SLC39A9,FLJ11274,MGC74989 pCMV3-ORFSpark- TAPL,ABCB9,KIAA1520,EST122234 pCMV3-ORFSpark- CATSPER2,HISPPD2A,KIAA0377,MGC51871,DKFZP313L0221 pCMV3-ORFSpark- LDH1,LDHA,LDH-M,PIG19 pCMV3-ORFSpark- ADIP,SSX2IP,FLJ...
for his unreserved encouragement and provision of constructive comments and guidance in the development of this research paper. Also their deepest gratitude goes to Shashemene hospital administrative and staff members, especially those who work in the card room for t...
His Tag Information A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a ...
FLAG 标签从 N-到 C-末端的多肽序列为:DYKDDDDK(1012Da)。它可以与其他亲和标签一起使用,例如多聚组氨酸标签(His 标签),HA 标签或 Myc 标签。它也可以融合到蛋白质的 C-或 N-端。一些商业抗体(例如 M1 / 4E11)仅在其存在于 N-末端时才能识别表位。然而,其他可用的抗体(例如 M2)则对位置不敏感。