穿梭载体(sbuttle vector)指在两种宿主生物体内复制的载体分子,因而可以运载目的基因(穿梭往返两种生物之间)。穿梭质粒含原核和真核生物 2 个复制子,以确保两类细胞中都能扩增。 科淼生物的各种产品发货前经经过严格的多重验证,收到质粒后请短暂离心,加入20ul ddH2O溶解质粒,取2ul转化至对应感受态中,挑取单克隆重新...
英文别名: PCDH-CMV-MCS-EF1-GFP+PURO vector 中文名: PCDH-CMV-MCS-EF1-GFP+PURO载体 中文别名: PCDH-CMV-MCS-EF1-GFP+PURO载体 CBNumber: CB25775114 分子式: 分子量: 0 MOL File: Mol file PCDH-CMV-MCS-EF1-GFP+PURO载体化学性质 安全信息 ...
英文名称 PCDH-CMV-MCS-EF1-GFP+PURO vector 英文同义词 PCDH-CMV-MCS-EF1-GFP+PURO vector CAS号 分子式 分子量 0 EINECS号 相关类别 Mol文件 Mol File 结构式 PCDH-CMV-MCS-EF1-GFP+PURO载体 性质 PCDH-CMV-MCS-EF1-GFP+PURO载体 用途与合成方法 安全信息 MSDS信息 PCDH-CMV-MCS-EF1-GFP+PURO...
pCDH-CMV-MCS-EF1-RFP-T2A-Puro载体基本信息:载体名称: pCDH-CMV-MCS-EF1-RFP-T2A-Puro 质粒类型: 慢病毒表达载体;cDNA表达载体;双启动子载体克隆方法: 多克隆位点,限制性内切酶 启动子: CMV 载体大小: -- 5' 测序引物及序列: CMV-F :CGCAAATGGGCGGTAGGCGTG 3' 测序引物及序列: -- 载体标签: 无 ...
The packaging vector provides all the proteins essential for transcription and packaging of an RNA copy of the expression construct into recombinant viral particles. To produce a high titer of viral particles, expression and packaging vectors are transiently co-transfected into producer mammalian cells ...
SBI第三代慢病毒载体 SBI offers a third generation of the most popular HIV-1 based lentivector expression system which consists of three maincomponents: (1) The lentiviral expression vector (e.g., pCDH-EF1-MCS-T2A-Puro) (2) The lentiviral packaging plasmids (e.g., pPACKH1 Packaging Plasmid...
and expression of the target gene sequence. The packaging vector provides all the proteins essential for transcription and packaging of an RNA copy of the expression construct into recombinant viral particles. To produce a high titer of viral particles, ...
pCDH-CMV-MCS-EF1-GFP+Puro 载体信息 产品货号GS-8070 销售价格¥ 1224 包装规格2μg质粒或0.5ml甘油菌 筛选标记Puromycin 载体大小8196 bp 报告基因copGFP 启动子CMV pCDH-CMV-MCS-EF1-GFP+Puro 载体图谱
The packaging vector provides all the proteins essential for transcription and packaging of an RNA copy of the expression construct into recombinant viral particles. To produce a high titer of viral particles, expression and packaging vectors are transiently co-transfected into producer mammalian cells ...
In this study a conserved sequence of ORFV- DNA Pol was synthesized and cloned in pCDH-CMV-MCSEF1-cGFP-T2A-Puro lentiviral vector at the upstream of GFP gene. The validity of cloning was confirmed by restriction enzyme digestion and sequencing. Therefore, this recombinant plasmid will be ...