Five microliters of each sample were analyzed on a 1.2% agarose/ethidium bromide gel, were extracted with a QIAquick gel extraction kit (Qiagen), and were cloned into Escherichia coli through use of pGEM®–T Easy Vector (Promega). DNA was purified from minipreps using QIAquick miniprep ...
Fig. 6: Mutation of hPCDHA9 results in its instability and abnormal FAK phosphorylation. WT or mutant hPCDHA9 in pCMS.EGFP vector were transfected in HEK293 cells as indicated (a–h).aWestern blot of WT and mutant hPCDHA9 levels. GFP was used as transfection efficiency controls.bConfocal ...
we first constructed KPNB1-silenced cell lines in Panc-1 and BxPC-3 cells. Next, we overexpressed PCDH1 or control vector in KPNB1-silenced cells and measured the level of nuclear localization of p65 by Western blot. Compared with the shNC groups, we found that the ability of PCDH1 to p...
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual Clone information Clone Map MSDS Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 2017-07-01 Organism Danio rerio(zebrafish) Product protocadhe...
Plasmid description:Mammalian Expression vector of Homo sapiens protocadherin gamma subfamily C, 4 with N terminal Flag tag. Selectable markers:Neomycin Map: 产品分类:真核过表达质粒 订购信息 Catalog numberPlasmid nameSizePrice BC101035pcDNA3.1-Flag-PCDHGC42ug/20ul 小提质粒询价 ...
Plasmid description:Lentiviral shRNA Knockdown vector of Homo sapiens protocadherin gamma subfamily C, 4. Selectable markers:Puromycin Map: 产品分类:慢病毒shRNA质粒 订购信息 Catalog numberPlasmid nameSizePrice 56098pPLK/GFP+Puro-PCDHGC4 shRNA3个靶点 小提质粒询价 ...
c The corresponding heat map kymograph shows the relative velocity of the mesoderm roughly every 5 min. d Thick solid (dashed) lines are smoothed average profiles of wild-type (WT; Pcdh18a deficient) embryos, with individual embryo profiles shown in lighter lines in ϕ (red/orange) and θ...
1.2 × 105/well for RT-PCR and Western blots. Neurons were maintained in complete medium at 37 °C, 5% CO2. Neurons were infected at days in vitro (DIV)0 with adeno associated viral (AAV) particles expressing GFP-Cre (AAV9.hSyn.HI.eGFP-Cre.WPRE.SV40, Penn Vector, USA; 5....
Plasmid description:Mammalian Expression vector of Homo sapiens protocadherin beta 3 with N terminal Flag tag. Selectable markers:Neomycin Map: 产品分类:真核过表达质粒 订购信息 Catalog numberPlasmid nameSizePrice BC104817pcDNA3.1-Flag-PCDHB32ug/20ul 小提质粒询价 ...