Early replicative intermediates with newly synthesized leading strand, approximately 1 kilobase pair long, immediately downstream of the replication origin accumulate in products synthesized in extracts from a dnaT strain that lacks primosomal protein i or in wild-type extracts supplemented with anti-...
Eucaryotic replication origin binding proteins[J]. Front Biosci, 2004, 9 :2133-2143. [32]Kowalski D, Natale DA, Eddy MJ, et al. Stable DNA unwinding, not"breathing, "accounts for single-strand-specific nuclease hypersensitivity of specific A+T-rich sequences[J]. Proc Natl Acad Sci U S...
Bolivar and co-workers engineeredpBR322as acloning vectorseveral years back (Bolívar et al., 1977a,b). One of its most outstanding features is that it contains the uni-directionalreplication origin ofthe naturalColE1plasmid. As a consequence, the 2D gel patterns generated by the RIs of pBR...
This chapter discusses pBR322, which is is a 4,362-bp double-stranded DNA plasmid cloning vehicle designed to allow simple and rapid preparation of cloned recombinant DNA fragments. It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites for...
α‐Santonin, a compound extracted from the flower heads of Arthemisia maritima plant, was effective in elimination of small, multicopy, relaxed plasmids (pBR322 and pBR329 with CoLE 1 origin of replication in Escherichia coli , whereas plasmids of IncF 1 , H 1 and X group were totally ref...
ColE1-derived plasmid replication origin 2534-3122 pBR322 DNA (4361bp)的图谱如下: pBR322 DNA中没有的酶切位点(Restriction enzymes that do not cut pBR322 DNA)包括: Acc65I AflII AgeI AleI ApaI AscI AsiSI AvrII BaeI BbvCI BclI BglII BlpI BmgBI BsaXI BseRI BsiWI BsrGI BssHII BstBI Bs...
Because pBr322 and pUC19 both contain the same origin of replication, originally from pMBI (1), the bacterium does not distinguish between the two for purposes of replication. In addition, as the difference in size between the plasmids pUC19 and pBR322 is quite significant, with sizes of ...
pBR322 is an E. coli plasmid cloning vector containing the origin of replication from pMB1 (a plasmid in the ColE1 compatibility group; 1–3). The rop gene product, which regulates plasmid replication by stabilizing the interaction between RNAI and RNAII transcripts, maintains the copy ...
ColE1-derived plasmid replication origin 2534-3122 pBR322 DNA (4361bp)的图谱如下: pBR322 DNA中没有的酶切位点(Restriction enzymes that do not cut pBR322 DNA)包括: Acc65I AflII AgeI AleI ApaI AscI AsiSI AvrII BaeI BbvCI BclI BglII BlpI BmgBI BsaXI BseRI BsiWI BsrGI BssHII BstBI Bs...
pBR322plasmid.Inaddition,wesuggestedthat theSeqAproteincouldmodulateperiodicallytheinitiationofreplicationofthepBR322 plasmid.Thelatercouldbefixedbyitsoriginsequence,onahemimethylatedstate,during theinitiationofthereplication. CrownCopyrightÓ2010PublishedbyElsevierInc.Allrightsreserved. 1.Introduction Escherichia...