PBMCIsolation(BloodProcessing)TASCProtocol Label2tubes(serum),2tubes(DMSO),2tubes(FF–flashfrozen)with -patientnumber(PT#) -date -serum/DMSO/FF -patientblooddrawday# -#cells(forDMSOandFF) Keepbloodandcellsoniceasmuchaspossible. Sprayhoodandgloveswith70%EtOH Filllargebeakerwithbleach(undersink)and...
16.4 General Protocol 16.4.1 T Cell Isolation Protocols Because of the ease of access to peripheral blood, PBMCs, which contain T and B lymphocytes and monocytes, are the major source of human T cells used in most studies. There is a variety of available techniques for isolation and enrichme...
Human PBMC isolation and transfer Two batches of PBMCs were collected for animal and in vitro studies. The PBMCs adopted in the animal studies (Additional file 1: Table S1) and in the in vitro study (Additional file 1: Table S2) were collected from subjects who met the enrollment criteria...
Protocol for HIV-1 Isolation by PBMC Co-Culture July 2007Central, DukeIntroduction, I
Isolation of human PBMCs. Mononuclear cells were isolated according to Ficoll's protocol. Briefly, blood (5 ml) was mixed with cell medium (5 ml) supplemented with sole antibiotics and Ficoll medium (Life Sciences, Italy). Then, it was centrifuged at 1125 g for 20 minutes. ...
2.2Isolationoperipheralbloodmononuclearcells(PBMCs) usingFicoll-Paque™ ▲Tisprotocolisoptimized orthepreparationo PBMCs rom humanblood.Forthepreparationo PBMCs romrhesusmonkey (Macacamulatta)orcynomolgusmonkey(M.fascicularis)blood,it isrecommendedtouse96%Ficoll-Paque™. ...
Isolation of PBMC samples Venous blood samples were collected into heparin-coated tubes and centrifuged at 400×gfor 10 min at RT to remove platelet rich plasma. PBMCs were isolated by Ficoll Paque (Sigma Aldrich) density-gradient centrifugation and resuspended in CryoStor® CS10 (Stemcell), a...
Cell Isolation/Preparation: 1. Isolate peripheral blood mononuclear cells (PBMC’s) from whole blood following the published GE Healthcare protocol or the StemCell Technologies SepMate protocol. Notes for GE protocol: a. Whole blood should be isolated into anti-‐coagulant coated (sodium citrate...
3.1. Isolation and reprograming of PBMC, iPSC culture Peripheral blood was collected from patients after obtaining approval from the institutional ethics committee. PBMCs were isolated from blood using Ficoll-based density gradient centrifugation (Lymphoprep, SCT, 07801), frozen in CryoStor CS10 (SCT...
PBMC Isolation and Freezing using Vacutainer ® CPT Tubes for Flow Cytometry Assays PROCEDURE Materials • 8 mL Vacutainer ® Cell Preparation Tubes (CPT ™), with sodium heparin or sodium citrate (Becton Dickinson, Cat # 362753 or 362761)• 15 mL and/or 50 mL sterile polypropylene ...