PB全长2 472 bp，两端各有一个13 bp和19 bp的反向末端重复序列(inverted terminal repeat, ITR)，中间编码一个长594个氨基酸残基的转座酶。根据转座酶的转录方向来区分转座子的两个末端，两端最外侧各有长13 bp并且对称的ITR，内侧各有一段间隔区(spacer)，但并不对称(左端长3 bp，右端长31bp)...
end = strings.Repeat(" ", width-utf8.RuneCountInString(out)) }// and print! pb.mu.Lock() pb.lastPrint = out + end pb.mu.Unlock() switch { case pb.isFinish: return case pb.Output != nil: fmt.Fprint(pb.Output, "\r"+out+end) case pb.Callback != nil: ...
and grabs her drink and takes a gulp. Wash, rinse, repeat for most of the rest of the meal. One night I finally got it on video and now I present that to you.It still makes me smile. I just love how the S is missing and how she loves doing this over and over....
(PB印制电路板)PB专业 英译术语 PCB 专业英译术语 一、 综合词汇 1、 印制电路:printed circuit 2、 印制线路:printed wiring 3、 印制板:printed board 4、 印制板电路:printed circuit board (PCB) 5、 印制线路板:printed wiring board(PWB) 6、 印制元件:printed component 7、 印制接点:printed ...
开通VIP (PB印制电路板)PB专业 词汇 一、 综合词汇 1、 印制电路:printed circuit 2、 印制线路:printed wiring 3、 印制板:printed board 4、 印制板电路:printed circuit board (pcb) 5、 印制线路板:printed wiring board(pwb) 6、 印制元件:printed component 7、 印制接点:printed contact 8、 印...
plasmids with ωRNA was sequenced before packaging into AAV8 vehicle, and the AAV vectors were packaged by transfection of HEK293T cell with helper plasmids. The virus titer was 5 × 1013(AAV-TnpB-ωRNA) genome copies/mL as determined by qPCR specific for the inverted terminal repeat....
During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of ...
and one of the terminal hairpin arms at the inverted terminal repeats that constitute the viral replication origin15. The structure of the AAV5 OBD in complex with the direct repeat sequence has been reported (Fig. 7D)15. The arrangement of OBDs in this structure is not compatible with the...
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We note, however, that neither peptide appears to interact exclusively with one or the other BRCT repeat, consistent with the similarity in the primary amino acid sequence surrounding the two critical CDK sites (Figures 2A and 2C). Finally, we tested the importance of these phosphorylation sites...