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Thus, we used LC–MS/MS to metabolomics to identified significant alterations in macrophage metabolites. The CV diagram showed that the experimental data are very stable. We then performed the PCA before the content difference analysis, which indicating that there was distinct separation among OV-...
This is applied using metabolic pathway analysis mostly web-based software such as the MetaCyc database (MetaCyc.org) [63] and the MetaboAnalyst (metaboanalyst.ca) [64]. For example, MetaboAnalyst version 4.0 software [64] contains “omics” pathway libraries which help to indicate metabolic ...
Analysis of High Throughput (HTP) Data such as microarray and proteomics data has provided a powerful methodology to study patterns of gene regulation at genome scale. A major unresolved problem in the post-genomic era is to assemble the large amounts of
The Human Metabolome Database (HMDB, http://www.hmdb.ca/) was utilized to identify potential markers [22]. Finally, differential metabolites were analysed for metabolic pathway enrichment using MetaboAnalyst 5.0 (http://www.metaboanalyst.ca/) [23]....
The hypoxia response pathway enables adaptation to oxygen deprivation. It is mediated by hypoxia-inducible factors (HIF), which promote metabolic reprogram
Analysis using orthogonal projection to latent structures-discriminant s-plot (OPLS-DA S-plot) and MetaboAnalyst identified D-galactose and D-glucose as the significant contributors to sugar accumulation in the wucai plant. Interacting networks were mapped involving the 26 differentially expressed genes...
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Analyst 1.6.3 software was used to process the mass spectrometry data. Based on the local metabolic database, the metabolites of the samples were analyzed qualitatively and quantitatively by mass spectrometry. Primary pericyte isolation, culture and cell treatment Pericytes were isolated from C57/BL6 ...
MetaboAnalyst software (version4.0) was used to map metabolites that were associated with recurrence and survival in CRC to recognise metabolic pathways and identify genomic SNPs associated with CRC prognosis, referencing the following databases: Human Metabolome Database (HMDB), the Small Molecule ...