Milk protein secretion is of interest due to its physiological importance, from the point of view of the study of a high-efficiency secretory pathway and because of increasing interest in the use of transgenic manipulation of mammary gland gene expression to allow modification of milk composition, ...
Thus, we investigated whether TRPV1 activation can prevent the secretion of IL-6 from M1 macrophages. Using ELISA, we observed a massive secretion of IL-6 in the supernatant of M1 macrophage, which was significantly decreased by TRPV1 activation (Fig. 3D). Furthermore, we assessed the ...
STAT5-knockout mice present severe defects in mammary gland development and milk secretion, and STAT5b-/- mice exhibit defects in the production of GH.121 (2) Regulation of the immune system. STAT5 dimers are essential for survival, STAT5a- and STAT5b-deficient mice exhibit severe defects ...
The 30μg protein of samples was contained in SDS-PAGE. Then the proteins were transferred into PVDF membranes before blocked with 5% no-fat milk for 1 hour at room temperature. The PVDF membranes were incubated in antibodies' solution at 4 °C overnight. Next, the membranes were washed ...
LPS (1 μg/mL) significantly increased the secretion of IL-6, TNF-α, and IL-10, whereas YSYZ-F significantly inhibited the overproduction of these inflammatory factors in a dose-dependent manner (Fig. 4). YSYZ-F exerted more significant inhibitory effects on the production of IL-6 and ...
Enzyme preparations similar in their properties to those of mammary gland microsomes have been obtained by ultra-sonication of cytoplasmic droplets from freshly-secreted milk from goats. Enzymes of the sn -glycerol-3-phosphate pathway in milk have been confirmed through such preparations in vitro.DOI...
Silymarin, a polyphenolic flavonoid derived from milk thistle (Silybum marianum), is known to have anti-inflammatory, hepatoprotective and anticarcinogenic effects. In this study, the in vitro immunomodulatory effect of silymarin was investigated using human CD4+ T cells. Peripheral blood mononuclear cel...
et al. Autophagy promotes MSC-mediated vascularization in cutaneous wound healing via regulation of VEGF secretion. Cell death & disease. 2018;9:58 11. Xu X, Shao N, Duan X, Zhang X, Zhang Y. Tacrolimus alleviates Ox-LDL damage through inducing vascular endothelial autophagy. Eur Rev Med...
Besides, Arg-1 (M2 phenotype) mRNA and protein amounts were significantly increased after geniposide treatment, as well as IL-10 secretion. Conclusion These findings suggest that geniposide could inhibit the progression of and stabilize atherosclerotic plaques in rabbits by suppressing M1 macrophage ...
They demonstrated that exposure of human renal fibroblasts to high glucose significantly increased FN secretion, but the kinetics of high glucose-increased secretion was significantly delayed compared to that of TGF-β1-induced FN. Type III collagen was up-regulated by high glucose, but not by TGF...