KCNQ2/3 heterodimers are the main molecular basis for M-channels in neuronal cells, a voltage-dependent potassium channel. The channel is activated near the neural threshold potential and exhibits a non-inactivating, voltage-dependent, outward potassium ion profile. It is regulated by many neurotrans...
Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium influx in THP-1 macrophages. We describe steps for differentiating, resting, and activating THP-1 macrophages and then outline the procedures for whole-cell patch-clamp recordings....
Patch clamp is an electrophysiological technique used in neuroscience to record and measure the flow of ion currents across biological membranes through ion channels at the whole-cell or single-channel level. AI generated definition based on:Methods in Cell Biology,2002 ...
Assay Drug Dev Technol 1:685–693 CAS PubMed Google Scholar Liem LK, Simard JM, Song Y, Tewari K (1995) The patch clamp technique. Neurosurgery 36:382–392 CAS PubMed Google Scholar Mathes C (2003) Ion channels in drug discovery and development. Drug Discov Today 8:1022–1024 Pub...
A missing factor in chip-based patch clamp assay: gigaseal The ''gold'' standard in the study of ionic currents across biological membranes is the Patch Clamp method. However, this is a slow, labor and skill intens... WL Ong,L Yobas,WY Ong - 《Journal of Physics Conference》 被引量:...
1. Automated patch clamp (APC) electrophysiology has been increasingly adopted in research laboratories studying ion channels over the last two decades, due to the vastly increased throughput compa...
All sites used the consensus voltage clamp protocols, although one site applied pulses at a faster frequency (0.2 Hz vs. 0.1 Hz). Eight of nine sites stored test articles in glass (one using plastic). Four of the sites added only one addition of each concentration to the assay wel...
Whole-cell voltage clamp electrophysiology using glass patch pipettes (1) is regarded as the gold standard for measurement of compound activity on ion channels. Despite the high quality of the data generated by this method, in its traditional format, patch clamping has limited use in drug screenin...
BrdU assay for neurogenesis in rodents doi:10.1038/nprot.2006.224J Martin WojtowiczNohjin KeeNature ProtocolWojtowicz JM, Kee N. BrdU assay for neurogenesis in rodents. Nat Protoc. 2006; 1 (... JM Wojtowicz,N Kee - 《Nature Protocols》 被引量: 414发表: 2006年 Imaging synaptic vesicle exocyto...
High-throughput screening of drug candidates for channelopathies can greatly benefit from an automated patch-clamping assay. Automation of the patch clampi... Wee-Liat,Ong,Kum-Cheong,... - 《Lab on A Chip》 被引量: 50发表: 2007年 Microfluidic integration of substantially round glass capillarie...