Intercellular differences in ecDNA content drive expression differences The unequal mitotic segregation of ecDNA implies that ecDNA copy number can vary greatly between single cells17,22. In most single cells, multifragment ecDNAs did not differ in structure and composition (Fig. 3a,b), suggesting ...
美 英 un.并行组合 网络并联汇编;并联组合;平行组合 英汉 网络释义 un. 1. 并行组合 例句 释义: 全部,并行组合,并联汇编,并联组合,平行组合
Natural mitochondrial DNA (mtDNA) mutations enable the inference of clonal relationships among cells. mtDNA can be profiled along with measures of cell state, but has not yet been combined with the massively parallel approaches needed to tackle the compl
We searched for local similarity between each 500 kb sequence fragment from C. elegans against the genome of C. briggsae using MegaBlast (version 2.2.6) [46]. We performed MegaBlast searches with soft masking, e-value threshold of 0.001 and word seed size 100 bp (W100),...
So, in general, the search pattern is a tree. A fragment of the search tree for the example above is shown in Figure 4-3. Figure 4-3. Tree-shaped search pattern for the timetabling problem If we are interested in all the solutions (perhaps because we want to pick the best one ...
Examples of parallel computers include the multicore processors found in many computers today (even cheap ones), as well as many graphics processing units (GPUs).As an example of code for a shared-memory computer, below is a Java fragment intended to find the sum of all the elements in a...
Klenow Fragment (3’®5’ exo-) New England Biolabs Cat#M0212L KAPA 2x HiFi HotStart PCR mix Kapa Biosystems Cat#KK2602 Nextera XT Kit Illumina, Inc Cat#FC-131-1096 UltraPure DNase/Rnase-Free Distilled Water ThermoFisher Scientific Cat#10977015 TWEEN 20 Fisher Scientific Cat#BP337-100 Sodi...
A method of generating a massively parallel sequencing library, a) a step of preparing a primary WGA DNA library (pWGAlib) containing a fragment containing a WGA library universal sequencing adapter, and b) a single PCR cycle. A first, pWGAlib, comprising a first sequencing adapter (1PR5SA...
The fragment size of the 3′-end enriched sequencing library was analyzed by Qsep-100, and the average size was between 450 and 650 bp. The libraries were sequenced on the Illumina Nextseq 550 according to the manufacturer’s instructions, except that Custom read 1 was used for priming ...
This lack of parallel outliers could be because of the low number of markers used: 1030 amplified fragment length polymorphism markers and 112 single-nucleotide polymorphisms in the cichlid and whitefish studies, respectively. However, improved marker density does not always lead to the detection of ...