Specific modified para-nitrobenzyl esterases are disclosed which have improved stability and/or ester hydrolysis activity in aqueous or aqueous-organic media relative to the stability and/or ester hydrolysis activity of unmodified naturally occurring para- nitrobenzyl esterase.ARNOLD Frances H....
Then, by screening the clones expressing the enzyme plus a modified measure the ability to hydrolyze the substrate that was selected under the reaction conditions selected for these enzymes, to have the esterase activity of the enzyme is improved to identify. Para present unmodified, naturally - ...
A method for isolating and identifying modified para-nitrobenzyl esterases which exhibit improved thermal stability relative to naturally occurring para-nitrobenzyl esterase. The method involves preparing a library of modified para-nitrobenzyl esterase nucleic acid segments (genes) which have nucleotide ...
The para-nitrobenzyl esterase (PNBE) was successfully immobilized onto electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The maximum amount of PNBE immobilized on the PSPSMA nanofibers was determined to be 56.4 mg/g of ...
Specific modified para-nitrobenzyl esterases are disclosed which have improved stability and/or ester hydrolysis activity in aqueous or aqueous-organic media relative to the stability and/or ester hydrolysis activity of unmodified naturally occurring para- nitrobenzyl esterase.JEFFREY C. MOORE...
Cephalosporin p-nitrobenzyl esters are de-esterified via an esterase enzyme derived from the genus Bacillus.Donald Ray BrannonBrannon, D.R., J.A. Mabe and D.S. Fukuda. 1976. De-esterification of cephalosporin para-nitrobenzyl esters by microbial enzyme. J. Antibiotics 29: 121–124....
Specific modified para-nitrobenzyl esterases are disclosed which have improved stability and/or ester hydrolysis activity in aqueous or aqueous-organic media relative to the stability and/or ester hydrolysis activity of unmodified naturally occurring para-nitrobenzyl esterase.MOORE, JEFFREY, C....
PURPOSE: To obtain a new DNA compound useful for producing a large amount of PNB esterase in a recombinant host cell.CANTWELL CATHLEEN AキャスリーンアリスキャントウェルHODGES ROLAND LローランドレイマーホッジィズMCGILVRAY DEREKデレクマックギルブレイ...
Immobilization of the cross-linked para-nitrobenzyl esterase of Bacillus subtilis aggregates onto magnetic beads. Process Biochem. 45, 259- 263.Park, J.M,Kim M. et al.Immobilization of the cross-linked para-nitrobenzyl esterase of Bacillus subtilis aggregates onto magnetic beads.Process Biochemistry...
The invention provides purified PNB esterase from a microorganism of the genus . The enzyme is a monomer having a molecular weight of about 54,000 daltons. This PNB esterase catalyzes the deesterification of PNB-esters of cephalosporin and 1-carbacephalosporin compounds to the free acid form. ...