The assay was performed by incubating at different working temperatures (20, 30, 40, 50, 60, 70, 80, 90, and 100 °C) and using 0.1 M PBS with different pH values (3, 4, 5, 6, 7, 8, 9, and 10), adjusted using 0.1N HCl and 0.1N NaOH [39,52]. Each experiment was ...