The site-specific incorporation of nonstandard amino acids (nsAAs) during translation has expanded the chemistry and function of proteins. The nsAA para-azido-phenylalanine (pAzF) encodes a biorthogonal chemical moiety that facilitates “click” reactions to attach diverse chemical groups for protein ...
Orthogonal aminoacyl tRNA synthetase (aaRS)/tRNA pairs are an efficient tool for the site-specific introduction of para-azido-L-phenylalanine (pAzF), a non-canonical amino acid, into the amber codon of proteins. In the present study, to improve amber suppression by aaRS, random mutagenesis ...
Also provided are methods for incorporating the non-natural amino acid, para-methylazido-L-phenylalanine into a protein and further conjugating a biologically active adduct to the para-methylazido-L-phenylalanine.ZIMMERMAN Erik S.THANOS Christopher...
Also provided are methods for incorporating the non-natural amino acid, para-methylazido-L-phenylalanine into a protein and further conjugating a biologically active adduct to the para-methylazido-L-phenylalanine.Erik S. ZimmermanChristopher Thanos...
A composition comprising an aminoacyl-tRNA synthetase (RS), wherein RS: i) aminoacylates to an extent of more than 90% a tRNA with para-methylazido-L-phenylalanine (pAMF) compared to amino acids of common natural origin; ii) has a sequence identity of more than 80% with the tyrosyl ...