R脛NDLE: Immunoassay of insulin with insulin-antibody precipitate - HALES, J - 1963 () Citation Context ...ancreas of infected or uninfected mice and from freshly isolated beta cells by methods described elsewhere (15). The concentration of insulin from pancreas was measured by radioimmunoassay...
So LW-803 is effective not only for the analysis of aggregates (oligomers), which are polymer side impurities in antibody production, but also for analysis of low molecular components and monitoring of fragmentation. (Procedure of papain digestion) (1) Dissolve 3 mg of humanized monoclonal IgG ...
The presence of any brown colour is an indication of decomposition, and such preparations should not be used. Practical procedure The extent of digestion of mouse and rat IgG is highly dependent on the concentration of reducing agent. The procedure given below (Oi and Herzenberg, 1979) should ...
Briefly, proteome-derived peptide libraries were prepared by digestion of E. coli or HEK293 lysates with trypsin or GluC. These peptide samples (300 μg) were incubated with the enzyme to be tested (0.6–3 μg) in 100 mM sodium acetate (pH 4.5: NbCathB; pH 5.0: NbCysP6, NbCysP7, ...
The drugs containing papain have the functions of anti-cancer, anti-tumour, anti-lymphatic leukemia, anti-lysogenic bacteriums and anti-parasite, anti-tubercle bacillus and etc. It can diminish inflammation lidan pain helps digestion, treatment women's illnesses, glaucoma, osteoprolife...
into the rear footpads. A test bleed after 4 weeks yielded an antiseL'um which did not react with the resistant form, but did react with whole yG immunoglobulin and with the Fab, Fc fragments obtained by papain digestion of the whole AG immunoglobulin in the absence of cysteine (Fig. 1)...
into the rear footpads. A test bleed after 4 weeks yielded an antiseL'um which did not react with the resistant form, but did react with whole yG immunoglobulin and with the Fab, Fc fragments obtained by papain digestion of the whole AG immunoglobulin in the absence of cysteine (Fig. 1)...
The major transplantation antigen of the PVG rat, Rt-1 c, has been isolated from PVG leukaemic cells in milligram quantities for use in biological experiments related to transplantation. A 3-stage isolation procedure was developed involving the preparation of plasma membranes, digestion of the ...
To construct a tetracycline (tet)-regulated expression plasmid for SARS-CoV PLpro-TM, cDNA fragment encoding PLpro-TM along with its downstream V5-His6 tags in pCDNA3.1-SARS-PLpro-TM were excised by restriction digestion with HindIII and PmeI, and ligated into pTRE2Bla (39) that was ...
Bennett, KL, Smith, SV, Truscott, RJ, Sheil, MM (1997) Monitoring papain digestion of a monoclonal antibody by electrospray ionization mass spectrometry. Anal Biochem 245: pp. 17-27Bennett KL, Smith SV, Truscott RJ, Sheil MM (1997) Monitoring papain digestion of a monoclonal antibody by ...