4、arms-pcr的灵敏度在1%,对于肿瘤组织样本能够满足检测要求,但是对于检测血浆或血液中的含量低于1%循环肿瘤细胞,灵敏度不够,不能够及时对临床用药产生的耐药性突变及时进行检测,且受荧光通道的限制,具有检测目的数量少、低通量等不足的缺点,难以实现同一管内多个靶标的检测。 5、数字pcr用于等位基因突变检测的分析灵...
方法:收集2016年3月至2017年6月就诊的进展期NSCLC患者85例,采用PAP核酸扩增实时荧光PCR法与扩增阻滞突变系统(amplication refractory mutation system, ARMS)实时荧光PCR法检测血浆cfDNA的EGFR突变状况,比较组织与血浆及PAP法与ARMS-PCR检测结果的一致性;对突变阳性的38例患者采用PAP动态监测血浆基因突变状况。结果:血浆...
Performance comparison of real-time Bi-PAP and real-time ARMS PCR in the detection of template DNA derived from frozen tissue and FFPE tissue samples.Najie, SongXueting, ZhongQingge, Li
结论:焦磷酸化激活聚合反应(PAP)可用于血浆cfDNA的EGFR基因突变检测,其检测结果与组织检测结果无显著差异,但较组织检测简便,快捷,微创,实时;与ARMS PCR检测结果无显著差异,但较ARMS PCR经济实惠,尤其是对于需要多次动态监测的患者,造成的经济负担轻.晚期非小细胞肺癌患者建议行血浆cfDNAEGFR基因突变动态监测,患者能从...
The limit of detection for the nuclear single copy gene HMBS and HPV was both set to 10 copies per PCR. Statistical methods The data were first analysed using a per-protocol approach, that is, including only women assigned to the two arms who complied with the protocol. The cumulative ...
Self-sampling and HPV test detected both CIN2 and CIN2+, while cytology detected mainly CIN3+ lesions (Table 1). No difference was seen between the two study arms with respect to the detection rate of CIN3, but the HPV arm identified four times as many CIN2 per woman screened. CIN2 ...
(amplication refractory mutation system,ARMS)实时荧光PCR法检测血浆cfDNA的EGFR突变状况,比较组织与血浆及PAP法与ARMS-PCR检测结果的一致性;对突变阳性的38例患者采用PAP动态监测血浆基因突变状况.结果:血浆与组织突变检测结果差异无统计学意义(P=0.092).PAP与ARMS-PCR突变捡测结果差异亦无统计学意义(P=0.210).血浆...