CT perfusion measurements for diagnosis of different pancreatic diseases.The contrast administration protocol shows a significant effect on reported blood flow.Study parameters are very heterogeneous between studies, showing a need for standardization#Quantitative meta-analysis validates CT perfusion measurements...
hpCAFs (approximately 106 cells) or 2/3 cryosections of pancreatic lesion tissue using Trizol (Invitrogen) according to manufacturer's instruction. cDNA synthesis for quantitative real-time PCR (qRT-PCR) was carried out with LunaScript RT Super Mix (NEB) according to the manufacturer's protocol...
Ten patients underwent surgery off protocol. The 12-month PFS was 67% (90% CI, 56.9-100); the median PFS and OS were 16.6 months (95% CI, 13.3-40.6) and 37.2 months (95% CI, 17.5-not reached), respectively. Baseline ctDNA levels were detected in 16 of 22 patients (73%) and ...
RNA isolation was performed as suggested in the Tuveson Laboratory Murine and Human Organoid Protocols, briefly the culture media was aspirated away, and the Matrigel domes were dissolved into TRIzol Reagent (Thermo Fisher), followed by the RNA isolation protocol recommended by the manufacturer. Librar...
12 Because of its frequency, the term “pancreatic cancer” generally refers to ductal adenocarcinoma; this meaning will be used throughout this text, and ductal adenocarcinoma is the main topic of this protocol. The majority (60‒70%) of ductal adenocarcinomas arise in the head of the ...
The equipment used in these studies was CT multidetector (64– or 128–detector row scanners). All studies had to be performed with intravenous contrast according to each centre’s standard protocol, including arterial and portal venous phase imaging deemed optimal for accurate evaluation, with a ...
2.2. CEUS Study Protocol Patients were required to fast for at least eight hours prior to the scan, and all CEUS examinations were performed on the basis that the lesions can be clearly displayed on the corresponding gray-scale ultrasound (US). All US scans were carried out with ACUSON Sequo...
2.3. CEUS Study Protocol All patients fasted at least 8 h before ultrasound examinations. Ultrasound examinations were performed by an ACUSON Sequoia unit (Siemens Medical Solutions, Germany) equipped with a 3.5 MHz 6C-1 convex array transducer, or an ACUSON OXANA2 unit (Siemens Medical Solutions...
meaning that a detectable difference (beyond the contribution of noise in the system used to measure it) is found in the amount of expression of the genes relative to some baseline. In this case, the baseline is determined based on the algorithm. The genes of interest in the diseased cells...
The most widely used pancreatic cancer diagnostic tool is a multidetector CT angiography with a dual phasic pancreatic protocol. TheBiomedicinesarticle notes that a dedicated CT protocol for pancreatic lesions has been developed, citing a 90% specificity and sensitivity. “This protocol provides a stand...