Therefore, in this study, we aimed to determine the nucleoside transport properties of Panc-1 cells, one of the commonly used pancreatic adenocarcinoma cell lines. Materials and Methods: To assess the presence of equilibrative nucleoside transporter-1 (ENT-1) in Panc-1...
Re-expressionofCellAdhesionMoleculeInhibitsGrowthandInduces ApoptosisofHumanPancreaticCancerCellLinePANC--1 ZhiqingLIU(刘志清)’2十,LiangZHU(朱亮)H,Hua XiaoyuKE(柯晓煜),QiuZHAO(赵秋) QIN(覃华),DetainLI(李德民),ZuoqiXIE(谢作祁) DepartmentofGastroenterology,TongfiHospitalTongfiMedicalCollege,HuazhongUniversi...
PANC 1细胞分为三组( ① 未转染 PANC1 细胞、②转 染阴性对照siRNA、③ 转染siRNA PTTG)。 基因沉默 效应检查详见另文 [4] 。 1.2.2 TUNEL法检测细胞凋亡 细胞接种在预先 放有消毒盖片的六孔板中 ,基因沉默步骤同前。 转 染后48h 取出盖片 ,参照试剂盒说明书操作。 通过 显微图像分析系统进行凋亡细胞...
Cell culture and treatment The pancreatic cancer cell lines, Panc-1, BxPC-3, MIA PaCa-2, and the human pancreatic ductal epithelial cell line hTERT-HPNE were purchased from the Chinese Academy of Sciences Cell Bank of Type Culture Collection (CBTCCCAS, Shanghai, China). Panc-1 and MIA PaC...
cell viability of Panc-1 and MiaPaCa-2 cell lines with miR-30d mimics transfection.f,gColony formation assay was performed in two cell lines with miR-30d mimics transfection.hTranswell assays were performed to evaluate the effect of miR-30d on cell migration and invasion in Panc-1 and Mia...
2.3.1. Cell lines and cell proliferation assays The human pancreatic cancer cell line Panc-1 was purchased from Procell Life Science &Technology Co., Ltd. (Wuhan, China). The cell line was cultured in Dulbecco's modified eagle's medium (DMEM, Corning, USA) with 10% fetal bovine serum (...
The cell types that make up the pancreas are primarily ductal cells, acinar cells, and islet cells—any of which can give rise to a tumor, as well as primitive cells.12 Pancreatic cancer develops from previously normal cells through a build-up of genetic mutations, either inherited or ...
CD133+/CD44+/Oct4+/Nestin plus stem-like cells isolated from Panc-1 cell line may contribute to multi-resistance and metastasis of pancreatic cancer (Retra... Background and objective This study aims to investigate the role of Vitamin D (VD) in regulating the stemness and survival of CD13...
Douglas, J. E. et al. PIONEER-Panc: a platform trial for phase II randomized investigations of new and emerging therapies for localized pancreatic cancer.BMC Cancer22, 14 (2022). ArticlePubMedPubMed CentralGoogle Scholar Katz, M. H. et al. Borderline resectable pancreatic cancer: the importan...
Pancreatic ductal adenocarcinoma (PDAC) frequently metastasizes into the peritoneum, which contributes to poor prognosis. Metastatic spreading is promoted by cancer cell plasticity, yet its regulation by the microenvironment is incompletely understood. H