Cytokeratin isolated from bovine muzzle epidermis. 偶联物 Unconjugated 形式 Liquid 浓度 0.2 mg/mL 纯化类型 purified 保存液 PBS 内含物 0.09% sodium azide 保存条件 4°C, do not freeze 运输条件 Ambient (domestic); Wet ice (internat
Slides were incubated with primary antibodies (Supplementary Table 3)α-SMA (Abcam ab5694, 1:100 dilution), Ki67 (Thermo Fisher RM-9106-511, 1:500 dilution), pan-cytokeratin (Leica-Novostra C11, 1:50 dilution), PDGFR-β (Cell Signaling 3169 1:100 dilution), pMLC2 (Cell Signaling 3675S...
overnight at 4 °C. Immunocomplexes were recovered the following day with blocked 10 μl Protein G-Dynabeads (Thermo Fisher) for 2 h at 4 °C and washed twice with RIPA-low salt (10 mM Tris-HCl pH 8.0, 140 mM NaCl, 1 mM EDTA pH 8.0, 0.1% SDS and 0.1% Na-Deox...