PAMAM Dendrimer G1-NH2 PAMAM Dendrimer G2-NH2 PAMAM Dendrimer G3-NH2 PAMAM Dendrimer G4-NH2 PA...
1-Ethyl-3-(3-dimethylaminopro- pyl) carbodiimide hydrochloride (EDC) and N-hydroxysuc- cinimide (NHS) were purchased from Sigma (St. Louis, MO, USA). The sequence of Cdk1 siRNA: GCCAGA TAGTGGCCATGAATT (21 bp), and the sequence of Cdk2 siRNA: CUUCUAUGCCUGAUUAUAATT (21 bp). A ...
Materials used are trastuzumab (Herceptin®, Roche), polyamidoamine generation 3 (PAMAM G3.0) dendrimer with ethylene diamine core and amine surface groups, dimethylformamide or DMF (Sigma–Aldrich), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono (N-hydroxysuccinimide ester) or DO...
1-Ethyl-3-(3-dimethylaminopro- pyl) carbodiimide hydrochloride (EDC) and N-hydroxysuc- cinimide (NHS) were purchased from Sigma (St. Louis, MO, USA). The sequence of Cdk1 siRNA: GCCAGA TAGTGGCCATGAATT (21 bp), and the sequence of Cdk2 siRNA: CUUCUAUGCCUGAUUAUAATT (21 bp). A ...
百度爱采购为您找到6家最新的g2 pamam价格产品的详细参数、实时报价、行情走势、优质商品批发/供应信息,您还可以免费查询、发布询价信息等。
obtained from Sigma–Aldrich (USA). All other chemicals were of analytical grade. Double distilled water was used to prepare the solutions. 10mMphosphate-buffered saline (PBS), pH 7.0, was used for all measurements. The final concentrations of PAMAM dendrimers were 0.001, ...
For this purpose, PAMAM G3 dendrimer was conjugated with 16 celecoxib molecules (COX-2 inhibitor), 15 molecules of Fmoc-L-Leucine (agonist of PPARγ) and one tumor-targeting biotin molecule (G3-BCL) [39]. To evaluate the cytotoxic effect of the G3-BCL conjugate, three human cell lines...
Briefly, 10 mmol of 2-(dimethylamino)ethyl methacrylate (DMAEMA, Sigma-Aldrich, St. Louis, MO, USA) and 10 mmol of 1-bromohexadecane (BHD, TCI America, Portland, OR, USA) were combined with 3 g of ethanol in a 20 mL scintillation vial. The vial was stirred at 70 ◦C for 24 h...
PAMAM-G3 and its PEGylated counterpart were evaluated for lung cellular biodistribution. The compound presents a peak of concentration in systemic circulation within a few hours after pulmonary delivery in the presence or absence of PEG, presenting positive or negative charges, even with different ...
24-h prior to plating the cells, the coverslips were coated with 0.2 mg/mL Poly-L-Lysine (Sigma Aldrich, St. Louis, MO, USA). The cell viability was assessed using trypan blue (Thermo Fisher Scientific, Waltham, MA, USA) staining method. The cells were plated at a density of 2 × ...