The assay conditions for palmitoyl-CoA synthetase (P-CoA S) and carnitine palmitoyltransferase (CPT) in homogenates of human blood platelets have been studied. The assay based on trapping of palmitoyl-CoA by carnitine in the presence of exogenous CPT gave higher activity of P-CoA S than the ...
g. the determination of the activity of palmitoyl-CoA synthetase in rat liver microsomes, the acid-soluble CoA can be taken to be palmitoyl-CoA. In more complex systems it should be borne in mind that proteins can bind CoA. View chapter Book 1974, Methods of Enzymatic Analysis (Second ...
The presence of palmitoyl-CoA synthetase (EC 6.2.1.3) in the brush borderfree particulate fraction of chicken intestinal mucosa is demonstrated. The enzyme was dependent on the simultaneous presence of lysophosphatidylcholine and Triton X-100 as well as ATP, CoA and Mg 2+ for maximal activity. ...
coli cells for expression, gene acetyl coenzyme A synthetase was expressed engineering strain; (2) the gene engineering strain seed access fermentation initial initial culture medium; (3) After the initial culture was added to induce the induction culture medium was fed to produce acetyl coenzyme A...
M. ( 1975 ) Changes in the activities of de novo fatty acid synthesis and palmitoyl-CoA synthetase in relation to myelination in rabbit brain . Biochim. Biophys. Acta 380 , 165 – 175 .Cantrill, R. C., and Carey, E. M. 1975. Changes in the activities of de novo fatty acid ...
dna hybridizationThis study confirms assignment of the human long-chain acyl-CoA synthetase gene (FACL2) to chromosome 4 by analysis of somatic cell hybrid panels. The precise localization of this gene was determined to be in the 4q34-q35 region using FISH. 8 refs., 1 fig....
1. 1. The effects of palroitoyl-CoA on the structure and enzyme activity of the fatty acid synthetase from the insect Ceratitis capitata have been examined. 2. 2. The acyl-CoA derivative increases the helical content of the protein from 45 to 60% and inhibits the enzyme activity of the...
Tissue levels of long-chain acyl carnitine decreased suggesting that inhibition occurred at either fatty acyl CoA synthetase or carnitine-acyl CoA transferase. Cytosolic levels of acyl-CoA are low compared with mitochondrial levels and changes in acyl-CoA within the cytosolic compartment cannot be ...