运输条件:美国大陆的室温;其他地方可能有所不同。 产品活性:Palmitoyl coenzyme A lithium 是一种酰基-CoA 硫酯,可以通过肉碱穿梭系统转运到线粒体基质中,参与 β-氧化,也可以用作鞘氨醇生物合成的底物。 生物活性:棕榈酰辅酶 A 锂是一种酰基辅酶 A 硫酯,可通过肉毒碱穿梭系统转运至线粒体基质并参与 β-氧化。
Palmitoyl Coenzyme A is a chemical compound obtained from Sigma Chemical, St. Louis, MO, used in biochemical processes related to lipid metabolism. AI generated definition based on: Methods in Enzymology, 2000 About this pageSet alert Also in subject area: Nursing and Health ProfessionsDiscover oth...
All acyl-CoA derivatives were from Sigma. Cell culture supplies were from Life Technologies, Inc./BRL, except serum (Atlanta Biologicals). All other reagents were from reliable sources. Cell Lines and Membrane Preparations CHO-K1 cells (11) were maintained at 37°C in F-12 medium buffered at...
In addition, HFD increases pyruvate dehydrogenase kinase-4 (PDK4) activity and thereby downregulates the pyruvate dehydrogenase complex and thus the conversion of pyruvate into acetyl-CoA to be used in the Krebs cycle58. HFD also downregulates the glucose transporters within the CNS and increases ...
For L-carnitine treatment, once the tumors reached about 80 mm3, the mice were orally treated with L-carnitine at a dosage of 100 mg/kg per day (Sigma-Aldrich) or saline (200 μL) for 21 days (n = 6-7). Animal studies were conducted in accordance with institutional ...
CoA carboxylase 中文全称乙酰辅酶 A 羧化酶 ATGL adipocyte triglyceride lipase 脂肪甘油三酯脂肪酶 AKT2 Serine/threonine kinase 丝氨酸/苏氨酸蛋白激酶 BSA bovine serum albumin 牛血清白蛋白 cDNA complementary DNA 互补 DNA ChREBP carbohydrate response element 碳水化合物反应元件结合 binding protein 蛋白 FAT/...
Purified proteins were concentrated using Amicon Ultra filter units (Millipore-Sigma). Protein acyl transferase assay Purified zDHHC20 enzyme (50 nM) was assayed in a 50-μl reaction with [3H]-palmitoyl CoA (1 μM) and MBLAC2 (1 μM) at 25 °C for 0 to 30 min. The reaction was ...
Briefly, to assess activity, we used 50 μg of protein homogenized in Tris-HCl per liter (pH 9.2) and 5 mm DTT buffer per liter (Sigma-Aldrich). Samples were incubated with 200 μmol S-adenosyl-L-methionine per liter containing 0.5 μCi S-adenosyl-L-[methyl-3H]methionine (55.70 Ci/...
e,Preafpabalmocyci nS,Clu(t1e0o0li nm,Mpa)p, aPilnu,roDnNicasFe6I8 (bovine and all other biochemicals were purchased from Sigma-Aldrich (Milan, Italy) unless noted otherwise; Falcon tis- sue culture plasticware was purchased from BD Biosciences (SACCO srl, Cadorago (CO), Italy)...
Allyl vinyl ether 58 as a precursor of the [2,3]- Wittig rearrangement was prepared from 57 in a five-step sequence.43 Enolate formation by lithium N,N-diisopropylamide-induced sigma- tropic rearrangements as delineated in 59 to give tertiary alcohol ( ± )-60 in a racemic form, ...