Rac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 μg 产品介绍: 产品说明 一般描述 GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1 provided bound to glutathione agarose. Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell...
domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.Presentation PAK PBD Agarose beads, in color, are easy to visualize, minimizing potential loss during washes and aspirations of Rac-GTP pulldown (Figure 1).Figure 1: PAK-PBD Beads in Color ...
1 Pak1 的结构、激活和表达Pak1 是最早发现的 Paks 成员,包含 2 个重要的功能域: 1 个是在氨基末端的调节域即P21 结合位点( P21-bind-ing domain , PBD ),也称 Cdc42 / Rac1 结合域( Cdc42 / Rac1 in-teractive-binding , CRIB ), Cdc42 及 Rac1通过这个结构域与Pak1 结合;另 1 个是在羧基...
Description Rac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 µg Overview GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1 provided bound to glutathione agarose. Specifically binds to and precipitates Rac-GTP and cdc42-GTP fr...
The fact that filamin (a 280-kDa actin-binding protein) is known to bind the p21-binding domain (PBD) of PAK1 (aa52–132) forms a complex with PAK1 during HA/CD44-mediated extracellular signal-regulated kinase (ERK) signaling, suggests that a PAK1–filamin interaction could contribute ...
The PBD (p21-binding domain), AID (auto inhibitory domain), and kinase domain are shown. The PAK1Δ15 is lack of 15th exon (517-533 aa) thus has an incomplete kinase domain. h A375 and 451Lu cells were transfected with vector, FLAG-PAK1 or FLAG-PAK1Δ15 expression plasmids, and ...
domain of its partner molecule13,14. In this closed conformation, the kinase activity is very low. Binding of a small GTPase, like Rac1 or Cdc42, to the p21-binding domain (PBD) of PAK1 triggers conformation changes in the kinase domain, leading to dimer dissociation and to subsequent ...
与G蛋巍结合鳇PBD络稳域,鞋及数羹不等豹朦氦酸富集区[23-27]。基予PAKs 蛋自质绥构的保守性,将6种PAK辩构体分为两类;I类PAKs(PAKl--PAK3)和 II类PAKs(PAK4--PAK6)[28～30]。分析结构后发现I类PAKs存在几个保守的 结构域:l、PAKl的氨基末端,程转运、蛋白赝的折叠和倍母传导中发撵骥要 ...
effector domainGST-PAK-PBD pull-downAtypical RhoV GTPase (Chp/Wrch-2) is a member of the human Rho GTPase family, which belongs to the superfamily of Ras-related small GTPases. The biological functions of RhoV, regulation of its activity, and mechanisms of its ac...
To investigate the determinants of Cdc42-effector specificity, we utilized recombinant Cdc42 mutants and chimeras made between Cdc42 and RhoA to map the regions of Cdc42 contributing to specific effector p21-binding domain (PBD) interaction. Site-directed mutants of the switch I domain and ...