However, when fragment lengths vary and, in particular, when either the fragment size is shorter than a single-end read, or longer than twice the size of a single-end read, most state-of-the-art mergers fail to generate reliable results. Therefore, a robust tool is needed to merge ...
PEARis a paired-end reads merger for the Illumina platform. PEARevaluates all possible paired-end read overlaps and does not require the target fragment size as input. It also implements a statistical test for minimizing false-positive results. The highly optimized and parallelized implementation allo...
Motivation: The Illumina paired-end sequencing technology can generate reads from both ends of target DNA fragments, which can subsequently be merged to increase the overall read length. There already exist tools for merging these paired-end reads when the target fragments are equally long. However...
with default settings, but it was considerably slower thanEA-tools(28.2%∼31.6%of the speed);SeqPrep, a dedicated PE reads adapter trimmer and merger, had the highestmCC(0.9975) among the existing tools for processing PE reads, but it was slow (0.64Mbp/s);Btrimhad the highest speed (2...
mate of the error. Because the index read is short and sequenced earlier in the process, it likely has fewer errorsand,therefore,itserrorrateshouldunderestimate the true error rate. Regardless, the error rate specified for this step should not negatively affect the ability of ...
摘要: Motivation: The Illumina paired-end sequencing technology can generate reads from both ends of target DNA fragments, which can subsequently be merged to increase the overall read length. There alr ...关键词: Staphylococcus aureus Sequence Alignment Sequence Analysis, DNA Genomics Software High-...
PEAR: a fast and accurate Illumina Paired-End reAd mergeR Motivation: The Illumina paired-end sequencing technology can generate reads from both ends of target DNA fragments, which can subsequently be merged to in... J Zhang,K Kassian,F Tomáš,... - 《Bioinformatics》 被引量: 1197发表:...
The disclosure provides methods of generating paired reads in sequencing-by-synthesis process, particularly, in systems with relatively short read lengths (e.g., 15-35 bases), such as for example, in single molecule sequencing by synthesis. Several implementations of the methods are provided. Of ...