pag-tn5转座酶(transposase)是由tn5转座酶融合protein a、protein g和3×flag标签蛋白组成,可以更好的特异性识别抗体结合位点,加速tn5转座效率,并插入标签序列,最后通过高通量测序鉴定特定蛋白与dna互作。但是,pag-tn5转座酶存在着酶活不高,导致核小体特征峰不明显、宿主污染等问题,很大地制约了在cut&tag技术的...
ChipTag pAG-Transposase(6.5pmol/μL) 7.7μL CUT&Tag实验测试使用80000个细胞进行CUT&Tag实验,使用的二抗为山羊抗,如下图所示,获得的文库产量比pA-Tn5的多,使用pAG-Tn5代替pA-Tn5,可降低实验对二抗的要求。Lane B:阴性对照(不加一抗);Lane C:pA-Tn5 CUT&Tag扩增产物;Lane D:pAG-Tn5 CUT&Tag扩增产物;...
CUTANA Uncharged pAG-Tn5 is a fusion of Proteins A and G toE. colitransposase (Tn5) and is the key enzyme for CUT&Tag [1]. This product is highly purified to remove contaminatingE. coliDNA. For superior normalization, as well as antibody validation and reaction monitoring, SNAP-CUTANA...
Type:Transposase Mol Wgt:191 kDa Host:E. coli Epitope Tag:None 96/100120 Citations Description Available in 50 and 250 Reaction pack sizes. CUTANA pAG-Tn5 is the key reagent for performing efficient mapping of chromatin features in Cleavage Under Targets and Tagmentation (CUT&Tag) [Kaya-Okur...