In addition, the Ski8 subunit of the PAF complex is part of the hSKi complex that regulates RNA surveillance, suggesting an important function of the complex in coordinating events associated with proper RNA maturation during transcription (1,5). Jaehning, J.A. (2010) Biochim Biophys Acta ...
PAF1C associates with the integrator complex To elucidate the molecular mechanism behind PAF1C’s novel function in transcription termination on PTs, we first set out to identify proteins that associate with PAF1C. We established HeLa cell lines that stably expressed doxycycline (dox)-inducible, ...
The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. 仅用于科研。不用于诊断过程。未经...
CTR9(1–249)) and the minimal CTR9-binding region of PAF1 to a 60-residue fragment (aa 57–116, PAF1(57–116)) (Fig.1aand Supplementary Fig.1c–e). It was noted that CTR9(1–249)and PAF1(57–116)assembled into a complex since their co-elution in the analytical size-exclusion...
We show the Polymerase Associated Factor 1 Complex (Paf1C) is required to maintain multipotent progenitors of the neural crest (NC) lineage in zebrafish. Mutations affecting each Paf1C component result in near-identical NC phenotypes; alyron mutant embryos carrying a null mutation in paf1 were ...
The fission yeast is shown to have a mechanism to prevent small RNAs from inducing heterochromatin and epigenetic gene silencing; this protective model involves the highly conserved Paf1 complex, which is known to promote transcription and processing of
Histone Methylation RNA Polymerase II Structural Biology Transcription Elongation Factors X-ray Crystallography Cdc73 Paf1 Complex Parafibromin Ras Transcription Elongation Introduction Eukaryotic gene expression is facilitated by a large number of accessory factors that function at various steps in RNA synthe...
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Finally, we show that the Paf1 complex is required for Rad6-Bre1 catalytic activity but not for the recruitment of Rad6-Bre1 to promoters. Thus, in addition to its role during the elongation phase of transcription, the Paf1 complex appears to activate the function but not the placement ...
This role could be linked to its ability to bind to DNA complex structures as triplexes that may have function in regulation of gene expression.Aurélie Massoni-Laporte and Michel Perrot and Loïc Ponger and Hélian Boucherie and Anne-Laure Guieysse-PeugeotBiochimica et Biophysica Acta (BBA) ...