1a). In addition, chromatin immunoprecipitation coupled to qPCR (ChIP) revealed the localization of TBPL1 and PAF1 across the IGS (Fig. 1i, j), and this was comparable to other genetic loci known to be occupied by these factors (Supplementary Fig. 3b). The 28S rDNA coding region served...
While much is known about the regulation of larger chromosome domains in eukaryotes, the details of enhancer-promoter interactions remain poorly understood. Recent work suggests co-activators like BRD4 and Mediator have little impact on enhancer-promoter interactions. In leukemias controlled by the MLL-...
PAF1C has also been implicated in tumorigenesis3. Thus, subunits of PAF1C could act as oncogenes or tumor suppressors in a context-dependent manner. For example, the overexpression of PAF1 promotes pancreatic cancer formation32, and regulates self-renewal and drug resistance of pancreatic and ov...
Paf1C associates with RNA pol II throughout the coding regions of genes (26, 27) and engages in physical interactions with the elongation factors Spt16-Pob3/FACT and Spt4-Spt5/DSIF as well as RNA pol II (10, 13, 28, 29, 30). Consistent with its important functions during elongation...
Site-directed mutagenesis was used to introduce restriction enzyme sites into regions of Renilla luciferase coding sequence. The oligonucleotides used for amplifying the Flam, Tj 3′ UTR, mdg1, roo, 412, and blood elements are listed in Table S1. Genomic PCR fragments containing XmaI, KpnI, ...
Colour coding as in Fig. 3. (d) Position of the Cdc73 C-terminal GTPase-like domain (purple) contacting Rpb3. The crystal structure of the GTPase-like domain was docked into density of the correct size and oriented according to crosslinks to Rpb3 and Rpb11 (black lines). Paf1C ...
An expanded GGGGCC hexanucleotide of more than 30 repeats (termed (G4C2)30+) within C9orf72 is the most prominent mutation in familial frontotemporal degeneration (FTD) and amyotrophic lateral sclerosis (ALS) (termed C9+). Through an unbiased large-scale screen of (G4C2)49-expressing Drosop...
The coding sequence of p15PAF was amplified and sub-cloned into pcDNA3 next to an in-frame Myc tag allowing expression of C-terminally Myc-tagged p15PAF in mammalian cells. The full-length sequence of mouse p15PAF (KIAA0101) was cloned into pGEX4T-1. The entire coding sequence of p15PAF...
(Msm). The mutant was obtained by transformation ofMsm SMR5, the streptomycin resistant parent strain carrying a point mutation in the ribosomal protein S12 coding gene (rpsL) with the suicide plasmidpMCSΔpafBC-hyg-rpsLcarrying therpsL+gene and subsequent application of therpsLcounterselection ...