成分体积(μl)Sequencing Primer v21Elution Buffer32.3总体积33.3置于PCR仪上,80℃反应2 min,之后冰上放置。2)在200 μl低吸附管内混合文库与测序引物,此步骤需要通过软件Calculator计算,下载地址:github.com/PacificBiosc,举例如下。成分体积(μl)H2O0.9910× Primer Buffer0.89文库6Diluted Sequencing Primer0.98总...
them into 4 groups: 233 sense overlapping (17.44%), 392 sense intronic (29.34%), 295 antisense (22.08%), and 416 lincRNA (31.14%) (Fig.3c and d). The length of the lncRNA varied from 200 to 6384 bp, with the majority (54.87%) having a length ≤1000 bp, and mapped them t...
可以降低效应子功能的示例性氨基酸取代(根据eu编号方案)包括但不限于、c220s、c226s、c229s、e233p、l234a、l234v、v234a、l234f、l235a、l235e、g237a、p238s、s267e、h268q、n297a、n297g、v309l、e318a、l328f、a330s、a331s、p331s,或任何前述的组合。 糖基化可以有助于抗体的效应子功能,特...
Details regarding the qRT-PCR primers. Abbreviations JA: Jasmonic acid; SA: Salicylic acid; PacBio: Pacific biosciences; DETs: Differentially expressed transcripts; APA: Alternative polyadenylation; TFs: Transcription factors; ET: Ethylene; NPR1: Non-expressor of pathogenesis- related (PR) genes1; ...