p16INK4a Antibody (PA5-20379) in ICC/IF Immunofluorescence analysis of CDKN2A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minu...