TNF alpha Antibody (PA1-40281) in ICC/IF Immunofluorescence analysis of TNF-Alpha (Tumor necrosis factor-alpha) was performed using 70% confluent log phase RAW 264.7 treated with lipopolysaccharide (LPS) (10 ng/mL for 7 hr) and protein transport inhibitor cocktail [500X] (PTI) [1X for ...