The blot was probed with Anti-Vimentin Polyclonal Antibody (Product # PA1-16759, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 0.25 µg/ml, 1:4000 dilution). A 54 kDa band corresponding to Vimentin was ...
Vimentin Antibody (PA1-16759) in ICC/IF Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour ...