Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr573) Antibody (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower). Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phosph...
抗体名:Anti-Phospho-p90RSK (Thr573) 浓度:1mg/1ml % 靶点:详见说明书 免疫原:KLH conjugated Synthesised phosphopeptide derived from human p90RSK around the phosphorylation site of Thr573 宿主:详见说明书 应用范围:WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-50...
In addition, FMK and BI-D1870 repressed cell cycle regulatory genes cyclin D1 and Cdk4 induced by PDGF-BB, whereas inhibition of p90RSK augmented cyclin-dependent kinase inhibitor p27 downregulated by PDGF-BB in VSMCs, suggesting that p90RSK induces VSMC proliferation via cell cycle regulation...
RSK可以被活化的ERK1/2和3-磷酸肌醇依赖性蛋白激酶1(3-phosphoinositide dependent kinase 1,PDK1)直接磷酸化并激活,并且不受哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路的干扰,如图1所示。
cells, whereas wild-type and p53−/− cells were killed by this compound to a much lesser extent (Figure 2a), suggesting a close functional relationship between RSKs and p21. Figure 2 Effect of the RSK inhibitor BI-D1870 onγIR-induced stress responses. (a) BI-D1870 rescues HCT116 ...
Since p90RSK regulates the majority of the anti-apoptotic signaling of the ERK pathway, we investigated the effect of inhibiting p90RSK with its specific inhibitor BI-D1870 (BI-D) on apoptosis in CLL. Immunoblotting was carried out to investigate phosphorylation of components of the ERK/p90RSK...
P90RSK inhibitor BRD7389 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Primary antibodies against phospho-specific Erk1/2 (Thr202/Tyr204) (pErk), pan-Erk1/2, phospho-specific JNK (Thr183/Tyr185) (pJNK), pan-JNK, phospho-specific P38 (Thr180/Tyr182) (pP38), phospho-...
The p90 ribosomal S6 kinase (RSK) family comprises four mammalian Ser/Thr kinases (RSK1-4) (Romeo et al.2012). The first RSK family member was identified as a kinase activity in maturatingXenopus laevisoocytes that phosphorylated the 40S ribosomal subunit protein S6 (rpS6) (Erikson and Malle...
Huayan Huang1, Xiaokuan Zhu1, Yongfeng Yu1, Ziming Li1, Yi Yang1, Liliang Xia1* and Shun Lu1* Abstract Background Non-small cell lung cancer (NSCLC) patients with EGFR mutations exhibit an unfavorable response to immune checkpoint inhibitor (ICI) ...
Furthermore, phosphorylation levels of RLC20 and MYPT1 were both significantly decreased. Experiments involving the irreversible MLCP inhibitor microcystin-LR, in the absence of Ca2+, revealed that the decrease in phosphorylation levels of RLC20 upon RSK inhibition are not due solely to the increase...