M.SQSTM1介导的NCOA4-LC3相互作用在体内得到了证实,当SQSTM1K420R突变体共转染时,这种相互作用显著减少,揭示K420的泛素化对NCOA4和LC3在体内的相互作用至关重要。N.NCOA4和SQSTM1之间的相互作用在Erastin处理下显著增强,这可以通过...
为了进一步证明SQSTM1对铁蛋白吞噬的重要性,研究人员将野生型SQSTM1、SQSTM1ΔLIR或SQSTM1K420R重新转染到SQSTM1-/-MEF细胞,然后用厄拉斯汀处理。结果显示,野生型SQSTM1表达细胞中的NCOA4和FTL蛋白水平仅下降,而SQSTM1ΔLIR和SQSTM1K420R细胞中的NCOA4和FTL蛋白水平未下降;野生型SQSTM1重组细胞中的内源性L...
Impaired autophagy promotes Inflammatory Bowel Disease (IBD). Claudin-2 is upregulated in IBD however its role in the pathobiology remains uncertain due to its complex regulation, including by autophagy. Irrespective, claudin-2 expression protects mice f