Furthermore, we demonstrate that ATG2A and ATG9A lipid transferase activity regulates the rate of p62 condensate degradation. Finally, we show in CRISPR knock-in cell lines that ubiquitin is required for recruiting ATG9A to p62 condensates. Taken together, our data...
but its spatiotemporal regulation remains largely unknown. In a recent paper published inCell Research, Feng et al. show that branched actin filaments and Myo1D collect stochastically formed nanoscale p62 bodies and grow them into micron-scale condensate, which is prerequisite...
NEMO promotes p62 condensate formation at aggregates by lowering the threshold for ubiquitin-induced phase transition We and others recently observed that NEMO undergoes phase separation upon binding to linear ubiquitin chains50,79. Since Q330X NEMO is impaired in binding to M1-linked ubiquitin, it ...
Therefore, FAM134B and ATL3 are the two critical ER-phagy receptors involved in ORF8/p62 condensate-induced ER-phagy dysfunction. ORF8/p62 condensates facilitate viral DMV biogenesis by inhibiting ER-phagy The ER has been shown to serve as the main membrane donor for SARS-CoV-2 DMVs.16,46...
Cigarette smoke extract, containing 40 mg/ml condensate and 6% nicotine, was purchased from Murty Pharmaceuticals (Lexington, KY), and was prepared by smoking University of Kentucky's 3R4F Standard Research Cigarettes on an FTC Smoke Machine [15]. The smoke on the filter is calculated by the ...
As the liquidity of condensate is a critical determinant for its selective autophagy47,48, we studied the effect of celastrol on the liquidity of Nur77/p62 condensates. Fluorescence recovery after photobleaching (FRAP) analysis of co-transfected GFP-Nur77 and mCherry-p62 revealed that the ...
Alterations in cellular ubiquitin (Ub) homeostasis, known as Ub stress, feature and affect cellular responses in multiple conditions, yet the underlying mechanisms are incompletely understood. Here we report that autophagy receptor p62/sequestosome-1 int
condensate decontaminate machin/BEKOMAT13 BEKO TECHNOLOGIES GmbH condensate decontaminate machin/BEKOMAT12 BEKO TECHNOLOGIES GmbH damageable set/XEKA13101 BEKO TECHNOLOGIES GmbH damageable set/XEKA12101 DELTA F Corporation 153-1000 Nr:DF-17342-V6 WIKA A-10/0-40bar/4-20Ma/8-30V HERZOG 8-5504-344442-...
The channel wall forms or carries a removable condensate collection unit. An adjustable cleaning device near the heat exchanger inlet side removes fluff from the heat exchanger. The washer-dryer has a heat exchanger (20) in the process... HASELMEIER, RALF,BERNER, DIETRICH,LOOS, MARITA,... 被...
but its spatiotemporal regulation remains largely unknown.In a recent paper published in Cell Research,Feng et al.show that branched actin filaments and Myo1D collect stochastically formed nanoscale p62 bodies and grow them into micron-scale condensate,which is prerequisite for their autophagic ...