Human NOP53 qPCR Primer Pair,即人NOP53 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
Arrows indicated primer sets for ISCU1 (gray arrow), ISCU2 (black arrow), and common ISCU (white arrow). qPCR analysis of ISCU in U373MG (p53 mutant) cells infected with adenovirus expressing p53 (Ad-p53) or LacZ (Ad-LacZ) at a multiplicity of infection (MOI) of 10 or 20 (lower...
The qRT-PCR and ChIP-qPCR primer sequences and antibodies used in this study are listed (see Additional file 1: Supplemental methods). siRNAs, anti-sense oligo transfection, and PINTtransient overexpression All siRNAs and ASOs used in this study are listed (see Additional file 1: Supplemental ...
Western blot and quantitative real-time PCR (qPCR), respectively, revealed that DNMT1 and DNMT3β protein and mRNA expression levels were both significantly up-regulated in the H6+24 h group compared with the H0+24 h group (P < 0.01) (Fig. 3a,b), but that DNMT1 and DNMT...
(G) qPCR analysis of mtDNA copy number. (H-I) Comet assay to assess DNA damage. (J-K) Flow cytometry analysis to detect cellular apoptosis. (L) Western blot analysis of the impact of PQQ and MSC-Mito on the ATM/p53 signalling pathway in vitro. Scale bars: 10 μm. Values are the...
Western blot and quantitative real-time PCR (qPCR), respectively, revealed that DNMT1 and DNMT3β protein and mRNA expression levels were both significantly up-regulated in the H6+24 h group compared with the H0+24 h group (P < 0.01) (Fig. 3a,b), but that DNMT1 and DNMT...
Primers for PCR and qPCR, see Table S1 Primer Bank and this paper N/A PRMT1 siRNA QIAGEN Cat# SI00441861 and SI02663493 p53 siRNA QIAGEN Cat# SI01456511and SI01456525 Negative control siRNA QIAGEN Cat# 1022076 Software and Algorithms Partek Flow Partek Inc. N/A rMATS Kim et al., 2013...
3A–D). To assess the effect of GAS5 on p53 signaling pathway, p53 downstream genes BAX, PUMA, NOXA and p21 mRNA expression were analyzed by RT-qPCR in VSMCs transduced with AdGFP or AdGAS5. As shown in Fig. 3E, overexpression of GAS5 increased the mRNA expression of all the p53 ...
PCR primers for the p53-target genes were generated using the Universal Probe Library (Roche). All qPCR primer pairs are shown in Supplementary Table 11. Allele-specific expression analysis of the TP53β-stop-lost variant was performed using 2 µl 25x diluted cDNA as template for the ...
The oligonucleotides include domain-focused sgRNA library, exon-tiling sgRNA library, sgRNA sequences targeting the EP400 complex and p53 pathway, primers for qPCR with reverse transcription, ChIP–qPCR primers, shRNA primers and primers used for library preparation. Related to all figures. ...