p53 gene mutation is a late event in the progression of prostate cancer and is associated with advanced (metastatic) stage, loss of differentiation, and the transition from androgen-dependent to androgen-independent growth. Testing of prostate cancer biopsy specimens from metastatic sites for p53 ...
In addition to germline p53 mutations, the gene is the most commonly mutated gene in human cancers, with as much as 50% of tumors containing somatic p53 mutations. This unit provides a protocol to perform germline mutation analysis of the gene. The protocol includes steps for amplification and...
and to predict lung cancer recurrence.Methods PCR-SSCP technique was used to detect mutation of p53 gene at the resection margin of the bronchus (no cancer cells remained by gross pathological examination),cancer tissue and normal tissue of the lungs in 50 ...
Germ-line transmission of a mutated p53 gene in a cancer-prone family with Li|[ndash]|Fraumeni syndrome TUMOUR suppressor genes, whose usual function seems to be controlling normal cell proliferation 1,2 , have been implicated in many inherited and sporadic f... S Srivastava,Z Zou,K Pirollo...
TP53 mutation status and gene expression profiles are powerful prognostic markers of breast cancer Introduction Gene expression profiling of breast carcinomas has increased our understanding of the heterogeneous biology of this disease and promises to im... Anita Al Langerod,Hongjuan Hz Zhao,Ornulf ...
pylori status with both the free radical load and the presence of a p53 mutation. The hypothesis we have been testing here is that H. pylori, and in particular those strains that possess the cag A virulence gene, induces ROS in gastric tissue which subsequently introduces genetic alterations (...
Comments and Critique Predictive Testing for Germline Mutations in the ~53 Gene: Are All the Questions Answered? Comments and Critique Predictive Testing for Germline Mutations in the ~53 Gene: Are All the Questions Answered?THE EXPANDING FIELD OF THE MOLECULAR ......
The pattern of mutations that is now evolving is more complex than was suggested by the original reports, and includes a much wider spectrum of mutation types that occur throughout the gene. Furthermore, it is clear that germline p53 mutations are not restricted to families with classic LFS. ...
We have developed a fast single-strand conformation polymorphism (SSCP) technique to screen for mutations and polymorphisms in exons 5-8 of the human tumor suppressor gene p53. We use multiplex polymerase chain reaction (PCR) to amplify the four exons in one single PCR reaction and then ...
5.Wei-Wei Zhang, Longjiang Li, Dinggang Li, Jiliang Liu, Xiuqin Li, Wei Li, Xiaolong Xu, Michael J. Zhang, Lois A. Chandler, Hong Lin, Aiguo Hu, Wei Xu, and Dominic Man-Kit Lam.Human Gene Therapy.Feb 2018.160-179.