这种方法揭示了内质网驻留的孤儿P型泵P5A-ATP酶(酵母中的Spf1;人类中的ATP13A1)直接与线粒体尾锚定蛋白相互作用。由于遗传学研究已经将P5A-ATP酶(P5A-ATPase)与线粒体尾锚定蛋白错位联系起来,这些作者结合生化和结构方法来确定P5A-ATP酶的功能和机制。 3. Result 这些作者在无细胞系统中重建了膜蛋白插入细胞...
The most significant positive regulators identified included 尾2-microglobulin, a known regulator of MR1 surface expression, and ATP13A1, a P5-ATPase in the endoplasmic reticulum (ER) with putative transporter function not previously associated with MR1-mediated antigen presentation. CRISPR/Cas9-...
该研究发现P5A ATPase/CATP-8可以将错误定位到ER上的线粒体信号锚定蛋白(signal anchored,SA)和尾部锚定蛋白(tail anchored,TA)移除,维持ER特异“身份”。 研究人员以线粒体SA/TA蛋白为研究对象,在线虫中进行大规模筛选,发现catp-...
Here, we found that the orphan P5A-adenosine triphosphatase (ATPase) transporter ATP13A1 (Spf1 in yeast) directly interacted with the transmembrane segment (TM) of mitochondrial tail-anchored proteins. P5A-ATPase activity mediated the extraction of mistargeted proteins from the endoplasmic reticulum ...
P5A-ATPases exhibit a unique structural feature of P5A-ATPases described “arm-like domain” of unknown function. • The “arm-like domain” is not essential for ATP hydrolysis by the yeast Spf1p P5A-ATPase. • Deletion of the “arm-like domain” decreases autophosphorylation. Abstract...
上海玉博生物科技有限公司 主要致力于“Mouse monoclonal [M17-P5-F11] to beta Sodium Potassium ATPase Abcam ab2873 ”的生产销售。多年的“Mouse monoclonal [M17-P5-F11] to beta Sodium Potassium ATPase Abcam ab2873 ”生产与销售的经验,与各行业新老用户建立了
P5A-ATPase将错误定位的跨膜螺旋从内质网中移出,图片来自Science, 2020, doi:10.1126/science.abc5809。 P型ATP酶形成了一大类活跃的转运蛋白,存在于所有生命界,主要通过跨细胞膜运输离子或脂质。P5A-ATP酶属于真核生物特异性的P型ATP酶亚家族,它们的底物特异性是未知的。
A. (2012). Developmental expression of P5 ATPase mRNA in the mouse. Cell. Mol. Biol. Lett. 17, 153-170. doi: 10.2478/s11658-011-0039-3L.S. Weingarten, H. Dave, H. Li, D.A. Crawford, Developmental expression of P5 ATPase mRNA in the mouse, Cell. Mol. Biol. Lett. 17 (2012)...
这些作者使用了一种无偏见的、位点特异性的交联和质谱方法来鉴定这样的蛋白跨膜区。这种方法揭示了内质网驻留的孤儿P型泵P5A-ATP酶(酵母中的Spf1;人类中的ATP13A1)直接与线粒体尾锚定蛋白相互作用。 由于遗传学研究已经将P5A-ATP酶(P5A-ATPase)与线粒体尾锚定蛋白错位联系起来,这些作者结合生化和结构方法来确...
Objectives: ATPase Type 13A4 (ATP13A4) is a cation-transporting, P5-type ATPase that has been implicated in neurodevelopmental disorders. Our recent microarray study reported a significant increase in ATP13A4 mRNA levels in Brodmann's area (BA) nine in subjects with schizophrenia compared to ...