启动子: P43 promoter 复制子: p15A ori,repB 质粒大小: 5166bp 原核抗性: Amp 克隆菌株: DH5a 培养条件: 37度 表达宿主: 枯草杆菌 ■ 质粒属性 质粒宿主: 枯草杆菌 质粒用途: 蛋白表达 片段类型: 片段物种: 原核抗性: Amp 真核抗性: 荧光标记: ■ 质粒简介 pHY-P43载体是枯草杆菌表达质粒,P43启动...
启动子: P43 promoter 复制子: p15A ori,repB 质粒大小: 5166bp 原核抗性: 氨苄青霉素Amp 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃,有氧,LB 表达宿主: 枯草杆菌 规格:2ug 保质期:3个月 保存温度:-20℃ 使用说明 1、收到质粒干粉后请5000rpm离心1min ,再加入20ul ddH2O溶解质粒 2、取2ul质粒转化至10...
2008. Comparison of PaprE, PamyE and PP43 promoter strength for $\beta$ -galactosidase and staphylokinase expression in Bacillus subtilis. Biotechnol. Bioproc. Eng. 13: 313-318.JUNE-HYUNG KIM ET AL.: " Comparison of PaprE, PamyE, and Pp43 promoter strength for β-galactosidase and ...
枯草中可诱导表达系统:P43’+核糖开关 原文题目:Engineering an inducible gene expression system forBacillus subtilisfrom a strong constitutive promoter and a theophylline-activated synthetic riboswitch DOI:10.1186/s12934-016-0599-z 杂志:Microbial Cell Factorie...
transcription from RNA polymerase II promoter positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein kinase B signaling cellular response to lipopolysaccharide cellular response to cytokine stimulus negative regulation of neuron death positive regulation of actin cytoskeleton ...
The P43 promoter has been previously used for constitutive expression of exogenous genes within B.subtilis vectors 2. The context with which we used the promoter P43 is as a Polymerase Per Second (PoPS) generator.Vector Name: pHP13-P43 Antibiotic Resistance: Chloramphenicol Length: 5100 bp Type:...
[11]Gat O,Inbar I,Aloni - Grinstein R,et al. Use of a promoter trap sys- tem in Bacillus anthracis and Bacillus subtilis for the development of recombinant protective antigen - based vaccines[J]. Infection and Immunity,2003,71( 2) : 801 - 813.相关...
For complementation studies in yeast, the MAP kinase cDNAs for ntf6, ntf6-LOF,ntf6-GOF, and ntf4 were cloned by PCR into the yeast expression vector pVT103U (12), which contains the constitutive alcohol dehydrogenase promoter and a uracil-selectable marker. Oligonucleotides were designed to ...
P.375 Sequence variation in the enhancer 2/basal core promoter/precore region of hepatitis B virus and subsequent risk for hepatocellular carcinoma Lipopolysaccharide (LPS) is a key inflammatory mediator. It has been proposed to function as an important molecule that alerts the host of potential ba...
A nucleotide sequence encoding a foreign envelope protein of (a) a virus, the first promoter is operably linked to (b) the envelope coding sequence, the present invention comprises a nucleotide sequence coding for (c) antigen protein, relates to a vaccine composition comprising the recombinant ba...