Smac mimetic TL32711/birinapant (A-1901: used at 100 nM) (previously known as compound-C49) was purchased from Active Biochem (Maplewood, NJ, USA). CHX (239763: used at 10 μg/ml) was purchased from Calbiochem (San Diego, CA, USA). Plasmids Bacterial expression plasmid pGEX-GST-...
Immunodetection was performed using the follow- ing antibodies: mouse anti-HA monoclonal antibody clone 16B-12 (1:1000, Covance Research Products, Princ- eton, NJ), mouse monoclonal anti-PARP clone C-2-10 (1:1000, Oncogene Research Products, Boston, MA). mouse monoclonal anti-β-actin ...
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Smac mimetic TL32711/birinapant (A-1901: used at 100 nM) Cell Death Discovery (2017) 17023 Official journal of the Cell Death Differentiation Association (previously known as compound-C49) was purchased from Active Biochem (Maplewood, NJ, USA). CHX (239763: used at 10 μg/ml) was ...
The blots were washed three times in TBS-T, and immunoreactive bands were detected using Western Lighting Chemiluminescence Reagent Ultra (Perkin Elmer, Branchburg, NJ). References 1. Higgins, C. F. ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8, 67–113 (1992). 2. ...