(四)“A positive p16 immunostain supports the diagnosis of histologic HSIL if the morphological assessment of H&E slides is consistent with CIN 2 or CIN 3. There is a risk of overcalling cervical histology results when p16 is used incor...
(四)“A positive p16 immunostain supports the diagnosis of histologic HSIL if the morphological assessment of H&E slides is consistent with CIN 2 or CIN 3. There is a risk of overcalling cervical histology results when p16 is used incorrectly. Most importantly, a morphologic CIN 1 on H&E ...
The cutoff value for p16 immunostain in FNA specimens showing best results in our series is 10%. When p16 IHC is negative in FNA specimens, a repeat stain on a surgical specimen is recommended to avoid a false negative diagnosis.doi:10.1016/j.humpath.2016.04.002Xu, Bin...
Using pathological results as reference, the positive predictive value, sensitivity, specificity and accuracy of P16/Ki-67 double staining, P16 INK4α single staining and HR-HPV testing for screening of high-grade squamous intraepithelial neoplasia (HSIL) and cervical cancer were compared. Results...
We used PyrPeg to specifically stain the cores of neuritic plaques and tested the co-localization of microglia, astrocytes, and Aβ (Fig. 1D). Interestingly, we observed that the volume of PyrPeg co-localized with Iba1-positive cells was greater than with GFAP-positive cells (Fig. 1E, F...
24 h and probed for β-galactosidase activity using C12FDG substrate (scale bar = 400 µm).eAECIIs immunostained with SPC (red) and DAPI (blue).fFlow cytometry analysis of AECII measuring purity of isolation.Xaxis is measuring EPCAM for epithelial cell specificity andyaxis is ...
The human involucrin-specific MoMAb SY540 (Research Diagnostics, Inc., Flanders, NJ) was used at 1:100 dilution as a positive control in each experiment to test the overall quality of the immunostain procedure. A MoMAb specific for non-immune IgG1, κ isotype (PharMingen) was used as a...
p16INK4A is currently used as a 'positive' immunohistochemical marker for CIN, which is proposed to aid the identification of high-grade cervical lesions [9]. To evaluate the use of D2-40 in helping the diagnosis of CIN, we performed immunoreactivity of D2-40, compared to p16INK4A, on...
Figure 2. ISH for HPV (A, C, andE) and immunohistostaining for p16 (B, D, and F).A and B, C and D, and E andF are in the same areas of each lesion. A andB: HPV-16-positive cervical cancer case. A: ISH pattern showing the dot-type signals. B: Clear and distinct positiv...
intraepithelialneoplasia(CIN)issubjecttoahighlevel ofinterobservervariabilityandasubstantialnumberof false-positiveandfalse-negativeresults.Weassessed theimpactoftheconjunctiveinterpretationofp16 INK4a - immunostainedslidesontheaccuracyofcommunity- basedpathologistsindiagnosinghigh-gradecervical intraepithelialneoplasia(CIN...